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Purified anti-IRF9 Antibody

Pricing & Availability
Clone
5A3A39 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon regulatory factor 9 (IRF-9), Interferon-stimulated gene factor 3 gamma (ISGF-3 gamma), ISGF3 p48 subunit
Isotype
Mouse IgG2b, κ
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Product Citations
publications
1_5A3A39_Purified_IRF9_Antibody_WB_060614
Total lysates (15 µg protein) from Jurkat (lane 1), Jurkat treated with IFNα1 (lane 2), THP-1 (lane 3) and Raw264.7 cells (lane 4) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1250 diluted (0.4 µg/mL) Purified anti-IRF9 Antibody, clone 5A3A39 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-IRF9 Antibody or a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH Antibody. (lower). Lane M: Molecular weight ladder.
  • 1_5A3A39_Purified_IRF9_Antibody_WB_060614
    Total lysates (15 µg protein) from Jurkat (lane 1), Jurkat treated with IFNα1 (lane 2), THP-1 (lane 3) and Raw264.7 cells (lane 4) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1250 diluted (0.4 µg/mL) Purified anti-IRF9 Antibody, clone 5A3A39 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-IRF9 Antibody or a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH Antibody. (lower). Lane M: Molecular weight ladder.
  • 2_5A3A39_Purified_IRF9_Antibody_IF_060614
    IFN-α treated Jurkat cells were stained with purified anti-IRF9 (5A3A39) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody (Cat. No. 405310). Nuclei were stained with DAPI (blue).
  • 3_5A3A39_GoChIP_IRF9_Antibody_110617.png
    Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from THP-1 cells treated with IFNα2 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-IRF9 (Clone 5A3A39), or B) equal amount of Purified Mouse IgG2b, κ Isotype Control Antibody. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MX1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
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660702 100 µg 288 CHF
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Description

IRF9 is a member of the IRF family of transcription factors and plays an important role in IFN signaling pathways and anti-viral immune responses. Type I and type III interferon stimulation induces phosphorylation and heterodimerization of STAT1 and STAT2. The activated STAT1:STAT2 heterodimer recruits IRF9 and forms a heterotrimeric complex termed IFN-stimulated gene factor 3 (ISGF3). The ISGF3 complex, in turn, translocates to the nucleus and transactivates IFN-inducible genes. The IRF9 protein in the ISGF3 complex is responsible for the recognition of IFN-stimulated response elements (ISRE). Mice lacking IRF9 fail to induce the expression of IFN regulated genes in response to type I interferon stimulation, indicating that IRF9 is crucial in mediating interferon signaling.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human IRF9 recombinant protein (116-387 a.a.) expressed in E. coli.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified.
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, store undiluted 2°C and 8°C.
Application

WB - Quality tested
ICC, ChIP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.4 - 2.5 µg per mL. For ChIP applications, the suggested dilution is 1:50 to 1:200 by volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not react with mouse (in-house tested). A non-specific band was observed (around 37 KD) when this antibody was tested on Raw264.7 cells

Product Citations
  1. Xiang Q, et al. 2022. PLoS Pathog. 18:e1010676. PubMed
RRID
AB_2563575 (BioLegend Cat. No. 660702)

Antigen Details

Structure
393 amino acids, predicted molecular weight of 44 kD; contains a IRF tryptophan pentad repeat responsible for DNA binding.
Distribution

Cytoplasm, phosphorylated form translocated to the nucleus.

Function
IRF9 is a transcription factor that mediates interferon signaling and activates the expression of interferon inducible genes.
Interaction
Forms the interferon-stimulated gene factor 3 complex (ISGF3) with Signal Transducer and Activator of Transcription 1 (STAT1) and STAT2 in response to type I IFN stimulation.
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Fink K and Grandvaux N, 2013. JAKSTAT. 2:e27521.
2. Tsuno T, et al. 2009. J. Immunother. 32:803.
3. Thibault DL, et al. 2008. J. Clin. Invest. 118:1417.
4. Kraus TA, et al. 2003. J. Biol. Chem. 278:13033.
5. Xiao W, et al. 2001. J. Biol. Chem. 276:23275.
6. Veals SA, et al. 1992. Mol. Cell. Biol. 12:3315.

Gene ID
10379 View all products for this Gene ID
UniProt
View information about IRF9 on UniProt.org
Go To Top Version: 4    Revision Date: 08.24.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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