- Clone
- A19012A/R/C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Transcription factor Jun, activator protein 1 (AP1), Proto-oncogene c-Jun, transcription factor AP-1 subunit Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A19012A-R-C_PURE_Jun-phospho_Antibody_051222 A19012A-R-C_PURE_Jun-phospho_Antibody_051222](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A19012A-R-C_PURE_Jun-phospho_Antibody_051222-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 μg protein) from untreated (-) or UV-treated (+) NIH/3T3 cells were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:5000 dilution) of purified anti-Jun Phospho (Ser73) for 2 hours at room temperature (left). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Equal c-Jun loading was confirmed by probing membranes with anti-pan c-Jun (right). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a protein loading control at a 1:10000 dilution. Lane M: Molecular weight marker -
Whole cell extracts (15 μg protein) from UV untreated (-) or treated (+) HeLa cells were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.1 μg/mL (1:5000 dilution) of purified anti-Jun Phospho (Ser73) for 2 hours at room temperature (left). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a protein loading control at a 1:10000 dilution. Lane M: Molecular weight marker -
NIH/3T3 cells were treated with UV and rested for 35 minutes, fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and treated with lambda protein phosphatase (LPP) (right panel) or left untreated (left panel). Cells were then blocked with 5% FBS for 60 minutes and intracellularly stained with 5.0 µg/mL (1:100 dilution) of purified anti-Jun Phospho (Ser73) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) at 2.5 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective. Scale bar: 50 µm -
HeLa cells untreated (negative control, open histogram), or UV-treated (positive control, filled histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Purified anti-c-Jun Phospho (Ser73) (clone A19012A/R/C) or Purified Mouse IgG1, κ Isotype Control Antibody (open histogram, dashed line) (representative histogram for untreated or treated cells) (Cat. No. 400101) followed by Alexa Fluor™ 647 Goat anti-mouse IgG (Cat. No. 405322).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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605551 | 25 µg | 118 CHF | ||||
605552 | 100 µg | 293 CHF |
c-Jun is the first oncogenic transcription factor ever discovered. It heterodimerizes with members of the Fos protein family, such as c-Fos, to form the AP-1 transcription factor complex, exhibiting both activating and repressive functions depending on the interacting partner and promoter sequence context. c-Jun is required for cell cycle progression, and is protective against DNA damage, such as that resulting from ultraviolet light exposure. c-Jun is also overexpressed in many cancers including lung, breast and vulvar cancer, and is a target for anti-cancer therapies. Phosphorylation of c-Jun at serine 63 and 73 is activational, and mediated by the MAPK signaling cascade.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide of human c-Jun phosphorylated at Ser73
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 3.0 - 5.0 μg/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2924613 (BioLegend Cat. No. 605551)
AB_2924613 (BioLegend Cat. No. 605552)
Antigen Details
- Structure
- c-Jun is a 331 amino acid protein with a predicted molecular weight of 35.7 kD.
- Distribution
-
c-Jun is primarily expressed in the nucleus
- Function
- Transcription factor
- Interaction
- c-Jun forms the AP-1 transcription factor complex by heterodimerizing with proteins of the Fos family
- Biology Area
- Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Transcription Factors
- Molecular Family
- Phospho-Proteins
- Antigen References
-
- Pulverer B, et al. 1991. Nature. 353:670-4.
- Vogt P. 2001.Oncogene. 20:2365-77 .
- Brennan A, et al. 2020. J Exp Clin Cancer Res. 39:184.
- Gene ID
- 3725 View all products for this Gene ID
- UniProt
- View information about Jun Phospho Ser73 on UniProt.org
Related FAQs
Other Formats
View All Jun Phospho Ser73 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Jun Phospho (Ser73) | A19012A/R/C | WB,ICC,ICFC |
Alexa Fluor® 488 anti-Jun Phospho (Ser73) | A19012A/R/C | ICFC |
Alexa Fluor® 647 anti-Jun Phospho (Ser73) | A19012A/R/C | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Jun Phospho (Ser73)
Whole cell extracts (15 μg protein) from untreated (-) or U... Whole cell extracts (15 μg protein) from UV untreated (-) or... NIH/3T3 cells were treated with UV and rested for 35 minutes... HeLa cells untreated (negative control, open histogram), or ... -
Alexa Fluor® 488 anti-Jun Phospho (Ser73)
HeLa cells untreated (negative control, open histogram), or ... -
Alexa Fluor® 647 anti-Jun Phospho (Ser73)
HeLa cells untreated (negative control, open histogram), or ...
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