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Purified anti-Lamin A/C Antibody

Pricing & Availability
Clone
LASS2D9 (See other available formats)
Regulatory Status
RUO
Other Names
CMD1A, HGPS, LGMD1B, LMN1, LMNL1, MADA, PRO1
Isotype
Mouse IgG3, κ
Ave. Rating
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Product Citations
publications
LASS2D9_Purified_Lamin_A-C_Antibody_042219.png
Total cell lysates (15 µg total protein) from Daudi (reduced expression control), U87-MG, HeLa, HepG2, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Lamin A/C Antibody, clone LASS2D9, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Predicted LMNA expression data was obtained from Human Protein Atlas.
  • LASS2D9_Purified_Lamin_A-C_Antibody_042219.png
    Total cell lysates (15 µg total protein) from Daudi (reduced expression control), U87-MG, HeLa, HepG2, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Lamin A/C Antibody, clone LASS2D9, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Predicted LMNA expression data was obtained from Human Protein Atlas.
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615801 25 µg 101 CHF
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615802 100 µg 253 CHF
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Description

Lamins A and C are structural components of the lamina, a scaffold of proteins localized to the nuclear inner membrane, and contribute to nuclear membrane dynamics and chromatin structure. Mutations in the LMNA gene have been linked to multiple laminopathy disorders, including Hutchinson-Gilford progeria syndrome (HGPS), an autosomal dominant disorder characterized by the appearance of accelerated and dramatic aging. Patients with HGPS have a mutation in the LMNA gene that results in aberrant splicing and production of the toxic, truncated Lamin A protein progerin that functions in a dominant negative pattern.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
GST fused to C-terminal fragment of Lamin A (amino acid residues 422-664)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

LASS2D9 recognized both human and mouse Lamin A/C when tested by WB; WL4G10 does not recognize mouse Lamin A or C.

WL4G10 displayed a significantly higher affinity for human Lamin A/C compared to LASS2D9 when tested for WB.

During PD testing for ICC applications, this clone produced weak staining of PFA-fixed HeLa cells that had been permeabilized with methanol or Triton X-100. We therefore do not recommend this clone for the application.

RRID
AB_2810663 (BioLegend Cat. No. 615801)
AB_2810663 (BioLegend Cat. No. 615802)

Antigen Details

Structure
Lamin A is a 664 amino acid protein with a predicted molecular weight of 74 kD. Lamin C is a 572 amino acid protein with a predicted molecular weight of 65 kD.
Distribution

Ubiquitously expressed/Nucleoplasmic side of inner nuclear membrane

Function
Fibrous component of nuclear lamina, provides framework for nuclear envelope, may interact with chromatin
Cell Type
Neurons
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Nuclear Markers
Antigen References
  1. Machiels B, et al. 1996. J. Biol. Chem. 271:9249.
  2. Barton R and Worman HJ. 1999. J. Biol. Chem. 274:30008.
  3. Gruenbaum Y, et al. 2000. J. Struct. Biol. 129:313.
  4. Stierlé V, et al. 2003. Biochemistry 42:4819.
Gene ID
4000 View all products for this Gene ID
UniProt
View information about Lamin A/C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04.19.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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