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Purified anti-LBP Antibody

Pricing & Availability
Clone
Poly28638 (See other available formats)
Regulatory Status
RUO
Other Names
LPS-Binding Protein, Lipopolysaccharide Binding Protein, Lipopolysaccharide-Binding Protein, BPI Fold Containing Family D, Member 2, BPIFD2
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly28638_PURE_LBP_Antibody_1_102519
Western blot of purified anti-LBP antibody (Poly28638). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28638_PURE_LBP_Antibody_1_102519
    Western blot of purified anti-LBP antibody (Poly28638). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28638_PURE_LBP_Antibody_2_102519
    IHC staining of purified anti-LBP antibody (Poly28638) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28638_PURE_LBP_Antibody_3_102519
    IHC staining of purified anti-LBP antibody (Poly28638) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28638_PURE_LBP_Antibody_4_102519
    IHC staining of purified anti-LBP antibody (Poly28638) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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863801 25 µg 125 CHF
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Description

LPS-binding protein (LBP) is a protein that binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), which is a glycolipid present in the outer membrane of all Gram-negative bacteria. It is involved in the immunological response to infections of Gram-negative bacteria, together with bactericidal permeability-increasing protein (BPI). LBP also interacts with the CD14 receptor and enhances its association with LPS. LBP is associated with diseases including bacterial sepsis, and is related to cytokine release in response to bacteria in immune system.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant LBP expressed in E.coli.
Formulation
0.01 M PBS, pH 7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage, aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Heymann CJF, et al. 2022. J Bone Oncol. 36:100451. PubMed
  2. Heymann CJF, et al. 2021. Cancer Lett. 522:63. PubMed
RRID
AB_2820150 (BioLegend Cat. No. 863801)

Antigen Details

Structure
LBP is a 481 amino acid protein with an apparent molecular mass of ~53 kD.
Distribution

Tissue Distribution: Brain, kidney, lung, and immune system
Cellular Distribution: Extracellular, nucleus, and membrane

Function
Innate immune response
Interaction
LPS, BPI, CD14 receptor
Cell Type
Mesenchymal cells, Monocytes, Neurons
Biology Area
Bacterial proteins and Toxins, Cell Biology, Immunology, Neurodegeneration, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Pretorius E, et al. 2018. PLoS One. 13(3): e0192121.
  2. Sakura T, et al. 2017. Cardiovasc Diabetol. 16(1):62.
  3. Worthmann H, et al. 2015. J Neuroinflammation. 12:13.
Gene ID
3929 View all products for this Gene ID 16803 View all products for this Gene ID 29469 View all products for this Gene ID
UniProt
View information about LBP on UniProt.org
Go To Top Version: 1    Revision Date: 10.25.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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