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Purified anti-Mac-2 (Galectin-3) Antibody

Pricing & Availability
Clone
M1606B04 (See other available formats)
Regulatory Status
RUO
Other Names
Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
M1606B04_PURE_Mac-2_Galectin-3_Antibody_WB_062918
Cell lysate from HepG2 (lane 1), A549 (lane 2), PC3 (lane 3), HaCaT (lane 4), MCF-7 (lane 5), HeLa (lane 6) and NIH3T3 cell lines (lane 7) (15 µg protein for each) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-Mac-2 (Galectin-3) antibody (clone M1606B04) (upper). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated anti-rat-IgG secondary antibody for the anti-Mac-2 (Galectin-3) antibody (upper panel) or a 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower panel) antibody. Lane M: Molecular weight ladder.
  • M1606B04_PURE_Mac-2_Galectin-3_Antibody_WB_062918
    Cell lysate from HepG2 (lane 1), A549 (lane 2), PC3 (lane 3), HaCaT (lane 4), MCF-7 (lane 5), HeLa (lane 6) and NIH3T3 cell lines (lane 7) (15 µg protein for each) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-Mac-2 (Galectin-3) antibody (clone M1606B04) (upper). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated anti-rat-IgG secondary antibody for the anti-Mac-2 (Galectin-3) antibody (upper panel) or a 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower panel) antibody. Lane M: Molecular weight ladder.
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677301 50 µg 71 CHF
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677302 500 µg 258 CHF
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Description

Galectin-3 (galactose-specific lectin 3) is a unique member of the β-galactoside-binding lectin family molecule, which plays an important role in regulation of cell proliferation, differentiation, and tumor cell metastasis. Galectin-3 is mainly found in the cytoplasm, but it is also seen in the nucleus and can be secreted by the non-classical, secretory pathway. In general, secreted galectin-3 mediates cell migration, cell adhesion, and cell-cell interactions through its high affinity binding with galactose-containing glycoproteins on the cell surface. Cytoplasmic galectin-3 exhibits anti-apoptotic activity and regulates several signal transduction pathways, whereas nuclear galectin-3 has been associated with pre-mRNA splicing and gene expression. Recent studies also indicate the potential role of galectin-3 as a biomarker for acute and chronic heart failure.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant full-length human Galectin-3.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 1-5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Sharma M, et al. 2020. Circ Res. 127:335. PubMed
RRID
AB_2565901 (BioLegend Cat. No. 677301)
AB_2565901 (BioLegend Cat. No. 677302)

Antigen Details

Structure
250 amino acids with a predicted molecular weight of approximately 26 kD.
Distribution

Localized in the nucleus, cytoplasm, cell surface, or extracellular matrix. Expressed by tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, and dendritic cells.

Function
Regulate cell proliferation, differentiation, adhesion, and anti-apoptosis.
Interaction
DMBT1, ITGA3, ITGB1, CSPG4, LGALS3BP, LYPD3, CYHR1, and UACA.
Ligand/Receptor
Variety of ligands such as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, CD66, and etc.
Cell Type
Dendritic cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Adhesion Molecules
Antigen References

1. Kim HR, et al. 1999. Cancer Res. 16:4148.
2. Reljic R, et al. 2004. Immunol. Lett. 93:51.
3. Takenaka Y, et al. 2004. Mol. and Cell. Biol. 24:4395.
4. Joo HG, et al. 2001. J Leuko. Biol. 4:555.
5. Gudowska M, et al. 2014. Pol. Merkur. Lekarski. 37:348.
6. Xin M, et al. 2015. Biomed. Pharmacother. 69:179.
7. Filipe MD, et al. 2015. Clin. Chim. Acta. 443:48.
8. Funasaka T, et al. 2014. Glycobiology 24:886.

Gene ID
3958 View all products for this Gene ID
UniProt
View information about Mac-2 on UniProt.org
Go To Top Version: 4    Revision Date: 06.14.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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