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Purified anti-MAPK14 Antibody

Pricing & Availability
Clone
5A1-C5-F11 (See other available formats)
Regulatory Status
RUO
Other Names
Mitogen-Activated Protein Kinase 14 (MAPK14), cytokine suppressive anti-inflammatory Drug Binding Protein, PRKM14, PRKM15, p38, SAPK2A
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5A1-C5-F11_PURE_MAPK14_Antibody_1_012122
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-MAPK14 (clone 5A1-C5-F11) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • 5A1-C5-F11_PURE_MAPK14_Antibody_1_012122
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-MAPK14 (clone 5A1-C5-F11) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • 5A1-C5-F11_PURE_MAPK14_Antibody_2_012122
    IHC staining of anti-MAPK14 (5A1-C5-F11) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB) (Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm
  • 5A1-C5-F11_PURE_MAPK14_Antibody_3_012122
    ICC staining of purified anti-MAPK14 (clone 5A1-C5-F11) on HeLa cells. The cells were fixed and with 4% paraformaldehyde (Cat. No. 420801), permeabilized with ice-cold methanol, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 5.0 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
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602651 25 µg 118 CHF
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602652 100 µg 293 CHF
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Description

MAPK14 is a member of the p38 MAPK family of serine/threonine-specific kinases. MAPK14 plays a critical role in signal transduction, and upon activation, modulates the activity of diverse signaling families, such as the TNF, TGF, and EGFR pathways, and regulates diverse behaviors including inflammation, stress response, cell cycle, apoptosis, transcription and translation. MAPK14 is important for organogenesis, participating in the development of the heart and blood vessels. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human MAPK14 protein fragments
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For ICC, we recommend 4% PFA fixation followed by permeabilization with ice cold methanol. We do not recommend 4% PFA fixation followed by permeabilization with 0.5% Triton-X, or fixation/permeabilization with ice cold methanol alone.  

RRID
AB_2910477 (BioLegend Cat. No. 602651)
AB_2910477 (BioLegend Cat. No. 602652)

Antigen Details

Structure
MAPK14 is a 360 amino-acid protein with a predicted molecular weight of 41 kD.
Distribution

MAPK14 is widely expressed in the cytoplasm and nucleus of most tissues.

Function
MAPK14 is a serine/threonine kinase.
Interaction
MAPK14 phosphorylates myriad targets including transcription factors, other kinases, enzymes, and histone proteins.
Biology Area
Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Canovas B, Nebreda A. 2021. Nat Rev Mol Cell Biol. 22:346-366.
Gene ID
1432 View all products for this Gene ID
UniProt
View information about MAPK14 on UniProt.org
Go To Top Version: 1    Revision Date: 01.21.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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