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Purified anti-marmoset CD45 Antibody

Pricing & Availability
Clone
6C9 (See other available formats)
Regulatory Status
RUO
Other Names
LCA, T200
Isotype
Mouse IgG1
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Product Citations
publications
6C9_Purified_Marmoset_CD45_Antibody_FC_052413
Marmoset peripheral blood lymphocytes were stained with purified CD45 (clone 6C9) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 6C9_Purified_Marmoset_CD45_Antibody_FC_052413
    Marmoset peripheral blood lymphocytes were stained with purified CD45 (clone 6C9) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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250202 100 µg 171 CHF
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Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Common Marmoset
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Marmoset mononuclear cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen spleen sections1 and Western blotting1. The 6C9 antibody has been reported to not cross-react with humans, cynomolgus monkeys, or squirrel monkeys.

Application References

(PubMed link indicates BioLegend citation)
  1. Ito R, et al. 2008. Immunol. Lett. 121:116. (IHC, WB)
RRID
AB_2562119 (BioLegend Cat. No. 250202)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein
Distribution

Hematopoietic cells, except circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I and Thomas M. 1994. Annu. Rev. Immunol. 12:85.

Gene ID
100385061 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org
Go To Top Version: 2    Revision Date: 08.23.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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