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Purified anti-Mesothelin Antibody

Pricing & Availability
Clone
A15125D (See other available formats)
Regulatory Status
RUO
Other Names
Antigen recognized by antigen K1, megakariocyte potentiating factor (MPF), soluble MPF, mesothelin related protein
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A_A15125D_PURE_Mesothelin_Antibody_041117
Total cell lysates (15µg protein) from PC3 (negative control), HeLa and NIH3T3 were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.5 µg/mL purified anti- Mesothelin antibody (clone A15125D) and competitor’s antibody used at manufactures recommended concentration (upper) or loading control GAPDH (clone Poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for clone A15125D, and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody and GAPDH. Lane M is the MW ladder.
  • A_A15125D_PURE_Mesothelin_Antibody_041117
    Total cell lysates (15µg protein) from PC3 (negative control), HeLa and NIH3T3 were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.5 µg/mL purified anti- Mesothelin antibody (clone A15125D) and competitor’s antibody used at manufactures recommended concentration (upper) or loading control GAPDH (clone Poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for clone A15125D, and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody and GAPDH. Lane M is the MW ladder.
  • B_A15125D_PURE_Mesothelin_Antibody_072622
    IHC staining using purified anti-Mesothelin (clone A15125D) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated with (B) or without (A) 10 μg/mL of antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scalebar = 50 μM
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696302 100 µg 276 CHF
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Description

Mesothelin (MSLN) was initially cloned from a library of human pancreatic cancer cells. It was also identified by a monoclonal antibody, MAb K1, which recognized a glycoprotein present on the surface of mesothelial cells. The human MSLN gene encodes a ~71 kD precursor protein of 622 amino acids. The C-terminal glycosylphosphatidylinositol (GPI) anchor addition signal is removed (cleavage site: Ser598) and the latter replaced with a GPI anchor. The MSLN precursor is cleaved by furin at Arg295 into two products, a ~31 kD mature megakaryocyte potentiating factor (MPF residues Ser34 - Arg286) and a ~40 kD GPI-anchored membrane-bound mature mesothelin starting from Glu296. The 40 kD GPI-anchored mesothelin can be released by treatment with phosphatidylinositol-specific phospholipase C. Soluble mesothelin specifically binds to ovarian carcinoma cell line OVCAR-3, mediated by the cancer antigen 125 (CA125), a mucin-like glycoprotein on the surface of tumor cells. CA125 and mesothelin are co-expressed in advanced grade ovarian adenocarcinoma, and it has been speculated that CA125 might contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to mesothelin. Also, mesothelin and CA125/mucin 16 (CA125/MUC16) are co-overexpressed at the invading edges of pancreatic cancer tissues, and their expression correlates with poor survival rates. Mesothelin is highly expressed in several human cancers including malignant mesothelioma, pancreatic, ovarian, cholangiocarcinoma, and lung adenocarcinoma.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human recombinant mesothelin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Optimal concentration of usage is 0.1 - 0.5 µg/mL for WB. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. The optimal concentration should be determined by titration for each individual assay of interest.

Application Notes

Precursor: 70 kDa. Mature form (N-glycosylated): 46-48kDa.

Clone A15125D does not cross react with mouse (in-house tested). 

RRID
AB_2687055 (BioLegend Cat. No. 696302)

Antigen Details

Structure
The human MSLN gene encodes a ~71 kD precursor protein of 622 amino acids. The MSLN precursor is cleaved into two products, a ~31 kD mature megakaryocyte potentiating factor and a ~40 kD GPI-anchored membrane-bound mature mesothelin
Distribution

Mesothelial cells lining the pleura, peritoneum, and pericardium and tumor cells

Function
Cell adhesion; may promote cancer cell survival, proliferation, and metastasis; confers resistance to cytotoxic drug-induced apoptosis
Interaction
Cancer cells
Ligand/Receptor
CA125/MUC16
Biology Area
Cancer Biomarkers, Cell Adhesion, Cell Biology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Kojima T, et al. 1995. J. Biol. Chem. 270:21984.
2. Chang K and Pastan I. 1996. Proc. Natl. Acad. Sci. USA 93:136.
3. Rump A, et al. 2004. J. Biol. Chem. 279:9190.
4. Bharadwaj U, et al. 2011. Mol. Cancer 10:106.
5. Chen SH, et al. 2013. Sci. Rep. 3:1870.
6. Tang Z, et al. 2013. Anticancer Agents Med. Chem. 13:276.

Gene ID
10232 View all products for this Gene ID
UniProt
View information about Mesothelin on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 07.26.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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