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Purified anti-Mitofusin-1 Antibody (Previously Covance catalog# MMS-5021)

Pricing & Availability
Clone
1G3.B6 (See other available formats)
Regulatory Status
RUO
Other Names
Mitofusin-1, transmembrane GTPase MFN1, hfzo1, hfzo2, fzo homolog, mitochondrial transmembrane GTPase FZO-2, mitochondrial transmembrane GTPase Fzo-1, transmembrane GTPase MFN1, mitofusin 1
Previously
Covance Catalog# MMS-5021
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1G3dotB6_PUREAF_Mitofusin-1_Antibody_1_120518
IHC staining of purified anti-Mitofusin-1 antibody (clone 1G3.B6) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 20 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1G3dotB6_PUREAF_Mitofusin-1_Antibody_1_120518
    IHC staining of purified anti-Mitofusin-1 antibody (clone 1G3.B6) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 20 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1G3dotB6_PUREAF_Mitofusin-1_Antibody_2_120518
    Western blot of purified anti-Mitofusin-1 antibody (clone 1G3.B6). Lane 1: Molecular weight marker; Lane 2: 15 µg of human brain lysate; Lane 3: 15 µg of Alzheimer's disease human brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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814602 25 µL 106 CHF
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814601 100 µL 275 CHF
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Description

Mitofusin-1 is a transmembrane GTPase protein involved in mitochondrial fusion, also known as hfzo1 or MFN1.  Mitochondrial fusion and fission occurs in many cells and is important to mitochondrial morphology and function.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide sequence corresponding to amino acids 547-558 of the human mitofusin 1 protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 10 - 20 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).

RRID
AB_2564785 (BioLegend Cat. No. 814602)
AB_2564785 (BioLegend Cat. No. 814601)

Antigen Details

Structure
Expected MW: 86 kD
Biology Area
Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
Molecular Family
Mitochondrial Markers
Gene ID
55669 View all products for this Gene ID
UniProt
View information about Mitofusin-1 on UniProt.org
Go To Top Version: 4    Revision Date: 07.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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