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Purified anti-MOB1A Antibody

Pricing & Availability
Clone
W20004B (See other available formats)
Regulatory Status
RUO
Other Names
MOB Kinase Activator 1A
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20004B_PURE_MOB1A_Antibody_1_082021.png
Whole cell extracts (15 µg total protein) from HeLa, K-562, MOLT-4 and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 μg/mL of purified anti-MOB1A antibody (clone W20004B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker. Lysates from cell lines were loaded in order of predicted ascending MOB1A expression (source: Human Protein Atlas).
  • W20004B_PURE_MOB1A_Antibody_1_082021.png
    Whole cell extracts (15 µg total protein) from HeLa, K-562, MOLT-4 and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 μg/mL of purified anti-MOB1A antibody (clone W20004B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker. Lysates from cell lines were loaded in order of predicted ascending MOB1A expression (source: Human Protein Atlas).
  • W20004B_PURE_MOB1A_Antibody_2_082021.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of purified rat IgG2b, κ isotype ctrl antibody (Cat. No. 400602) or purified anti-MOB1A antibody (clone W20004B). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a rabbit control antibody against a separate epitope of MOB1A. Lane M: Molecular weight marker.
Cat # Size Price Quantity Check Availability Save
946701 25 µg 118 CHF
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946702 100 µg 293 CHF
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Description

MOB1 was first identified in yeast. MOB1 belongs to the Mps One Binder (MOB) co-activator protein family and is highly conserved from yeast to mammals. In humans, there are two genes that are highly homologous to yeast's MOB1: MOB1A and MOB1B. At the protein level, MOB1A and MOB1B are 95% identical. MOB1 is mainly located in the cytoplasm and is part of the Hippo pathway. The Hippo pathway was first identified in Drosophilia and is highly conserved in mammals. The pathway regulates cell contact inhibition, organ size control, and cancer development. In mammals, MOB1 is phosphorylated by MST1/2 and the activated MOB1 promotes LATS1/2 activation which in turn phosphorylates YAP1 and TAZ, causing them to be retained in the cytoplasm for ubiquitous degradation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human MOB1A protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not suitable for ICC. It failed to produce specific signal by ICC after fixing cells following three fix and perm methods (e.g. 4% PFA/methanol, methanol or 4% PFA/Triton X-100).

RRID
AB_2894533 (BioLegend Cat. No. 946701)
AB_2894533 (BioLegend Cat. No. 946702)

Antigen Details

Structure
MOB1A is a 216 amino acid protein with a predicted molecular weight of 25 kD.
Distribution

Nuclear and cytosolic

Function
Hippo pathway
Biology Area
Cell Biology
Antigen References
  1. Florindo C. et al. 2012. J Cell Sci. 125:3085-90.
  2. Hergovich A. 2011. Cell Signal. 23:1433-40.
  3. Hong A. et al. 2016. Nat Rev Gastroenterol Hepatol. 13:324-37.
  4. Stavridi ES. et al. 2003. Structure. 11:1163-70.
  5. Wilmeth L. et al. 2010. Mol Biol Cell. 21:380-92.
  6. Zeng Q. and Hong W. 2008. Cancer Cell. 13:188-92.
Gene ID
55233 View all products for this Gene ID
UniProt
View information about MOB1A on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08.20.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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