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Purified anti-mouse CCL17 (TARC) Recombinant Antibody

Pricing & Availability
Clone
A20096E.Rec (See other available formats)
Regulatory Status
RUO
Other Names
CC chemokine TARC, C-C motif chemokine 17, CCL17, chemokine (C-C motif) ligand 17, small inducible cytokine subfamily A (Cys-Cys), member 17, Small-inducible cytokine A17, T cell-directed CC chemokine, TARC, Thymus and activation-regulated chemokine
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A20096ERec_PURE_CCL17_FC_102224
GM-CSF generated bone marrow derived dendritic cells (BMDCs) were treated with 1X Monensin (Cat. No. 420701) for 16-24 hours. Cells were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. No. 426803) and intracellularly stained with purified anti-mouse CCL17 (TARC) recombinant (clone A20096E.Rec) (left) or purified rat IgG2b, κ isotype control (right) followed by anti-rat IgG PE. Cells were then stained with anti-mouse/human CD11b (clone M1/70) APC.
  • A20096ERec_PURE_CCL17_FC_102224
    GM-CSF generated bone marrow derived dendritic cells (BMDCs) were treated with 1X Monensin (Cat. No. 420701) for 16-24 hours. Cells were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. No. 426803) and intracellularly stained with purified anti-mouse CCL17 (TARC) recombinant (clone A20096E.Rec) (left) or purified rat IgG2b, κ isotype control (right) followed by anti-rat IgG PE. Cells were then stained with anti-mouse/human CD11b (clone M1/70) APC.
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167302 100 µg 329 CHF
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Description

CCL17 was initially isolated from phytohemagglutinin-stimulated PBMCs. CCL17 is constitutively expressed in thymus and, upon activation, in several cell types. CCL17-mediated recruitment of Th2 cells and CLA+ CD4+ T cells and play key roles in allergic diseases like atopic dermatitis, allergic asthma, allergic rhinitis, and allergic contact dermatitis. In addition, CCL17 has been detected in idiopathic pulmonary fibrosis. CCL17 and CCL22 secreted by DCs seem to mediate the recruitment of Tregs to sites of inflammation in patients with chronic hepatitis. In addition, CCL17 is present in advanced human and mouse atherosclerosis, and CCL17+ DCs accumulate in atherosclerotic lesions. CCL17 expressed by DCs limit the expansion of Tregs. CCL17 is upregulated by a combination of cytokines (TNFa, IL-4, and IFNγ) in bronchial epithelial cells; TGFβ1, TNFα, and IFNγ in the human keratinocyte cell line; and histamine and prostaglandin E2P in immature DC.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is  ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance in each application.

Antigen Details

Structure
Chemokine
Distribution

Upon activation, CCL17 is expressed by PBMCs, monocytes, dendritic cells, bronchial epithelial cells, smooth muscle cells, keratinocytes, and fibroblasts.

Function
CCL17 chemoattracts T cells, Th2 cells, and CLA+ CD4+ T cells. It plays a major role in allergic diseases. CCL17 can be upregulated by IL-4 in T cells and by a combination of cytokines in different cells.
Interaction
T cells, Th2 cells, CLA+ CD4 T cells, T (reg) cells, NK cells, NKT cells, and macrophages
Ligand/Receptor
CCR4 (CD194)
Cell Type
Dendritic cells, Epithelial cells, Fibroblasts, Monocytes
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Imai T, et al. 1997. J Biol Chem. 272:15036-42.
  2. McIlroy A, et al. 2006. Immunology. 117:507-16.
  3. Yogo Y, et al. 2009. Respir Res. 10:80.
  4. Stutte S, et al. 2010. P Natl Acad Sci USA. 107:8736-41.
  5. Oo YH, et al. 2010. J Immunol. 184:2886-98.
  6. Weber C, et al. 2011. J Clin Invest. 121:2898-910.
Gene ID
20295 View all products for this Gene ID
UniProt
View information about CCL17 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10.22.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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