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Purified anti-mouse CD200R3 Antibody

Pricing & Availability
Clone
Ba160 (See other available formats)
Regulatory Status
RUO
Other Names
CD200 R3, CD200RLb, OX-2 Receptor 3, CD200 receptor 3
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
1_Ba160_PE_1_102711.jpg
C57BL/6 peripheral blood leukocytes were stained with FcεRIα APC and CD200R3 (clone Ba160) PE (top) or rat IgG2b, κ PE isotype control (bottom).
  • 1_Ba160_PE_1_102711.jpg
    C57BL/6 peripheral blood leukocytes were stained with FcεRIα APC and CD200R3 (clone Ba160) PE (top) or rat IgG2b, κ PE isotype control (bottom).
  • 2_Ba160_PE_2_102711.jpg
  • 3_Ba160_Purified_CD200R3_Antibody_022019.png
    C57BL/6 mouse peripheral blood cells were stained with FcεRIα APC and CD200R3 (clone Ba13) at day 0 (left) 2 days post-intravenous injection of 10µg of LEAF anti-mouse CD200R3 (clone Ba160, middle) or LEAF mouse IgG2b, κ isotype control (right).
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142302 200 µg 218 CHF
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Description

CD200R3, also known as CD200RLb and OX-2 Receptor 3, is a disulfide-linked dimmeric CD200R-like receptor which belongs to immunoglobulin superfamily.  Its positively charged aminio acid lysinein associates with ITAM- or YxxM motif-bearing adaptor molecules such as DAP12, DAP10, FcRγ, and CD3ζ. CD200R3 functions as an activating receptor to regulate IgE independent immune response.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse primary basophils
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Depletion - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Ba160 recognizes circulating and bone marrow basophils; it also recognizes a subset of mast cells in the peritoneal cavity and skin.

A single intravenous administration of 30 µg of LEAF Ba160 to mice induces a dramatic reduction of basophil number in peripheral blood from day 1 to day 10. *Tested in the development stage.

RRID
AB_10915471 (BioLegend Cat. No. 142302)

Antigen Details

Structure
Immunoglobulin superfamily; disulfide-linked dimmer with positively charged amino acids in transmembrane; molecular weight is about ~38 kD or ~74 kD (reduced or non-reduced condition, respectively).
Distribution

Basophils in peripheral blood, bone marrow, spleen: mast cells in peritoneal cavity and skin.

Function
Transduce stimulatory signals.
Interaction
DAP12, DAP10, FcRγ, or CD3ζ
Cell Type
Basophils, Mast cells
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Voehringer D, et al. 2004. J. Biol. Chem. 52:54117.
2. Kojima T, et al. 2007. J. Immunol. 179:7093.
3. Sato K, et al. 2009. Blood 113:4780.

Gene ID
74603 View all products for this Gene ID
UniProt
View information about CD200R3 on UniProt.org
Go To Top Version: 3    Revision Date: 02.20.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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