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Purified anti-mouse CD226 (DNAM-1) Antibody

Pricing & Availability
Clone
TX42.1 (See other available formats)
Regulatory Status
RUO
Other Names
DNAM-1, PTA1 (Platelet and T cell activation antigen 1), TLISA1, LFA-1 associated molecule PTA-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
TX42.1_PURE_CD226_Antibody_1_012218
C57BL/6 mouse splenocytes were stained with CD8b.2 APC and purified CD226 (clone TX42.1, top) or purified rat IgG2a, κ isotype control (bottom), followed by anti-rat IgG2a PE.
  • TX42.1_PURE_CD226_Antibody_1_012218
    C57BL/6 mouse splenocytes were stained with CD8b.2 APC and purified CD226 (clone TX42.1, top) or purified rat IgG2a, κ isotype control (bottom), followed by anti-rat IgG2a PE.
  • TX421_PURE_CD226_Antibody_2_120417
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml CD226 (clone TX42.1) purified and CD3ε (clone 500A2) Alexa Fluor® 594 (green) overnight at 4°C, followed by 2.5 µg/ml of goat anti rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for 2 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
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133630 500 µg 370 CHF
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Description

Mouse CD226 (DNAM-1) is a 65 kd glycoprotein of a member of Ig super-family adhesion molecule containing 2 immunoglobulin-like domains. It is associated with leukocyte function associated antigen-1 (LFA-1) involving in TCR-mediated signal transduction for T cell proliferation and differentiation. CD226 is also involved in NK cell and T cell mediated cytotoxicity against certain tumors. CD226 (DNAM-1) is constitutively expressed on naïve CD8+ and CD4+ T cells, macrophages, and NK cells. It was reported that CD226 expression is up-regulated on Th1 cells, but down-regulated on Th2 and Th0 cells. CD155 and CD112 are the ligands for CD226.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse DNAM-1 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested 
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Dardalhon V, et al. 2005. J. Immunol. 175:1558
  2. Shirakawa J, et al. 2006. International Immunology 18(6):951-957
Product Citations
  1. Potempa M, et al. 2022. J Immunol. 208:1742. PubMed
  2. Flietner E, et al. 2022. Sci Rep. 12:10616. PubMed
RRID
AB_2728149 (BioLegend Cat. No. 133630)

Antigen Details

Structure
65 kd glycoprotein of a member of Ig super-family containing 2 immunoglobulin-like domains.
Distribution

Expressed on majority of CD8+ and CD4+ T cells, NK cells, monocytes/macrophages and a subset of B cells. Reported to up-regulated on Th1 cells, but down-regulated in Th2 cells.

Function
Mediate cellular adhesion, involved in LFA-1 co-stimulatory signal for T cell differentiation and proliferation.
Ligand/Receptor
CD112 and CD155
Cell Type
B cells, Macrophages, Monocytes, NK cells, T cells, Th1
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1 Tahara Hanaoka S, et al. 2006. Blood. 107:1491
2 Shibuya K et al. 2003. J. Exp. Med. 198:1829.

Gene ID
225825 View all products for this Gene ID
UniProt
View information about CD226 on UniProt.org
Go To Top Version: 0    Revision Date: 12.11.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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