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Purified anti-mouse CD254 (TRANCE, RANKL) Antibody

Pricing & Availability
Clone
IK22/5 (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor-related activation-induced cytokine, Receptor activator of NF-κB ligand (RANK L), Osteoprotegerin ligand (OPGL), Osteoclast differentiation factor (ODF), SOFA, TNFSF-11, CD254, RANKL
Isotype
Rat IgG2a, κ
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Product Citations
publications
IK22-5_Purified_090707
Mouse TRANCE transfected cells stained with purified IK22-5, followed by biotinylated anti-rat IgG and Sav-PE
  • IK22-5_Purified_090707
    Mouse TRANCE transfected cells stained with purified IK22-5, followed by biotinylated anti-rat IgG and Sav-PE
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510002 500 µg 136 CHF
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Description

CD254 is a 19 kD TNF superfamily member also known as TRANCE (TNF-related activation induced cytokine), RANK ligand, RANKL, TNFSF11, OPGL, and ODF. TRANCE is expressed on activated T cells and osteoclasts and has been implicated in the regulation of T cell and dendritic cell interactions as well as osteoclast differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
NSO-derived recombinant mouse TRANCE
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric analysis, the suggested use of this reagent is ≤1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The IK22/5 antibody has been reported to block the binding of RANK ligand-Fc to RANK. Additional reported applications (for the relevant formats) include: immunoprecipitation2, Western blotting2, and blocking of ligand binding1,2,4. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 510009-510014).

Application References

(PubMed link indicates BioLegend citation)
  1. Miyahira Y, et al. 2003. J. Immunol. 171:6344. (Block)
  2. Personal communication (Block IP WB)
  3. Gao Y, et al. 2007. J. Clin. Invest. 117:122.
  4. Kamijo S, et al. 2006. Biochem. Biophys. Res. Commun. 347:124. (Block)
  5. Fionda C, et al. 2007. J. Immunol. 178:4039.
  6. Haslam SZ, et al. 2008. Endocrinology. 149:2098. (Block) PubMed
Product Citations
  1. Fionda C, et al. 2007. J Immunol . 178:4039. PubMed
  2. Jiang L, et al. 2022. Front Immunol. 12:730438. PubMed
  3. Haslam S, et al. 2008. Endocrinology. 149:2098. PubMed
  4. Singh K, et al. 2018. J Immunol. 200:595. PubMed
  5. Lopes N, et al. 2017. EMBO Mol Med. 0.954861111. PubMed
RRID
AB_315550 (BioLegend Cat. No. 510002)

Antigen Details

Structure
TNF superfamily; 19 kD (Mammalian)
Distribution

Activated T cells, osteoclasts

Function
Regulates T and dendritic cell interactions, and osteoclast differentiation
Ligand/Receptor
RANK, OPG
Bioactivity
Activates JNK and NF-κB; involved in bone resorption; induces the expression of IL-1, IL-6, IL-12, IL-15 in dendritic cells
Cell Sources
Activated T cells, myeloid bone marrow precursors, stromal fibroblasts, synovial cells, osteoblasts
Cell Targets
Dendritic cells, osteoclasts
Receptors
ODFR, ODAR, TRANCE-R
Cell Type
Osteoclasts, T cells
Biology Area
Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Josien R, et al. 1999. J. Immunol. 162:2562.
3. Takayanagi H, et al. 2000. Nature 408:600.
4. Wong B, et al. 1999. Mol. Cell. 4:1041.

Regulation
Type II integral membrane protein processed by TACE for secretion; upregulated upon TCR engagement
Gene ID
21943 View all products for this Gene ID
UniProt
View information about CD254 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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