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Purified anti-mouse CD27 Antibody

Pricing & Availability
Clone
A18209B (See other available formats)
Regulatory Status
RUO
Other Names
T14, S152, Tp55, TNFRSF7
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A18209B_PURE_CD27_Antibody_122821
C57BL/6 mouse splenocytes were stained with purified anti-mouse CD27 (clone A18209B) (left) or purified rat IgG2a, κ isotype control (right), followed by goat anti-rat IgG FITC. Cells were costained with anti-mouse CD3 Brilliant Violet 421™
  • A18209B_PURE_CD27_Antibody_122821
    C57BL/6 mouse splenocytes were stained with purified anti-mouse CD27 (clone A18209B) (left) or purified rat IgG2a, κ isotype control (right), followed by goat anti-rat IgG FITC. Cells were costained with anti-mouse CD3 Brilliant Violet 421™
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110102 100 µg 112 CHF
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Description

CD27 is also known as S152 and T14. A member of the tumor necrosis factor receptor (TNFR) superfamily, CD27 is a 45 kD protein expressed on peripheral T cells, memory B cells, NK cells, and thymocyte subset. Through its ligand, CD70, CD27 plays a key role in T cell and B cell interactions. Additionally, ligation of CD27 on naïve T cells may be important in their maturation to effector cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD27 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone A18209B can compete with clone LG.3A10 on target cells.
Clone A18209B can also block binding of CD70 to CD27 in a cell-based binding assay.

RRID
AB_2910285 (BioLegend Cat. No. 110102)

Antigen Details

Structure
TNFR superfamily, 45 kD
Distribution

Peripheral blood T cells, memory B cells, NK cells, thymocytes subset

Function
Costimulatory signal for T and B cell activation, T cell development
Interaction
TRAF2, TRAF5, CD27-binding protein (Siva)
Ligand/Receptor
CD70
Cell Type
B cells, Lymphocytes, T cells, Thymocytes
Biology Area
Adaptive Immunity, Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Hintzen RQ, et al. 1994. Immunol. Today 15:307-11.
  2. Akiba H, et al. 1998. J. Biol. Chem. 273:13353-8.
  3. Kobata T, et al. 1995. P. Natl. Acad. Sci. USA 92:11249-53.
  4. Camerini D, et al. 1991. J. Immunol. 147:3165-9.
Gene ID
21940 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12.28.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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