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Purified anti-mouse CD28 (Maxpar® Ready) Antibody

Pricing & Availability
Clone
37.51 (See other available formats)
Regulatory Status
RUO
Other Names
Tp44, T44
Isotype
Syrian Hamster IgG
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Product Citations
publications
37.51_Purified_CD28_Cytof_060314.jpg
BALB/c mouse splenocytes stained with 151Eu-anti-CD28 (37.51). T lymphocytes (top) and B lymphocytes (bottom) are displayed in the analysis. Data provided by DVS Sciences.
  • 37.51_Purified_CD28_Cytof_060314.jpg
    BALB/c mouse splenocytes stained with 151Eu-anti-CD28 (37.51). T lymphocytes (top) and B lymphocytes (bottom) are displayed in the analysis. Data provided by DVS Sciences.
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102119 100 µg 106 CHF
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Description

CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
C57BL/6 mouse T-cell lymphoma EL-4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The CD28 antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity3,4, in vitro induction of thymocyte differentiation2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 102116).

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim)
  2. Cibotti R, et al. 1997. Immunity 6:245. (Costim)
  3. Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block)
  4. Nishio M, et al. 1996. J. Immunol. 157:4347. (Block)
  5. Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim)
  6. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
  7. Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim)
  8. Wang W, et al. 2007. J. Immunol. 178:4885. (Costim)
  9. Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim)
  10. Perchonock CE, et al. 2007. J. Immunol. 179:1768.
  11. Barbi J, et al. 2007. Blood 110:2215.
  12. Milpied P, et al. 2011. Blood 118:2993. PubMed
  13. Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed
  14. Crispin JC, et al. 2012. J. Immunol. 188:3567. PubMed
  15. Li CR, et al. 2014. J Immunol. 192:1425. PubMed
  16. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
Product Citations
  1. Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
RRID
AB_2563736 (BioLegend Cat. No. 102119)
Disclaimer

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure
Ig superfamily, 44 kD
Distribution

Thymocytes, CD4+, CD8+ peripheral T cells, NK cells

Function
Costimulates T and NK cells
Ligand/Receptor
CD80 (B7-1), CD86 (B7-2)
Cell Type
NK cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380.

Gene ID
12487 View all products for this Gene ID
UniProt
View information about CD28 on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Go To Top Version: 2    Revision Date: 07.21.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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