- Clone
- W21115A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD326, EGP40, MIC18, TROP1, KSA
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![a. W21115A_PURE_CD326_IHC-F_092423 a. W21115A_PURE_CD326_IHC-F_092423](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W21115A_PURE_CD326_IHC-F_092423.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of purified anti-mouse CD326 (Ep-CAM) (clone W21115A) on frozen mouse uterus section. C57BL/6 mouse frozen uterus section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the tissue was incubated without (panel A) or with (panel B) 5 µg/mL of purified anti-mouse CD326 (Ep-CAM) at 4°C overnight, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. The nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective. Scale bar: 50 µm -
IHC staining of purified anti-mouse CD326 (Ep-CAM) (clone W21115A) on formalin-fixed paraffin-embedded C57BL/6 mouse intestine tissue. Following antigen retrieval using 1X citrate buffer 1X (10 mM, pH 6.0) (Cat. No. 420901), the tissue was incubated without (panel A) or with (panel B) 1 µg/mL of purified anti-mouse CD326 (Ep-CAM) at 4°C overnight, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. The nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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114202 | 100 µg | 375 CHF |
EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant protein aa24-266
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-F - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemical staining on frozen tissue sections, a concentration range of 2.5 - 10.0 µg/mL is suggested. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 1.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_3097223 (BioLegend Cat. No. 114202)
Antigen Details
- Structure
- 40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
- Distribution
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Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types
- Function
- Mediates calcium-independent homophilic cell-cell adhesion
- Ligand/Receptor
- CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM
- Cell Type
- Embryonic Stem Cells, Epithelial cells
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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- Borkowski TA, et al. 1996. Eur J Immunol. 26:110-4.
- Bergsagel PL, et al. 1992. J Immunol. 148:590-6.
- Gene ID
- 17075 View all products for this Gene ID
- UniProt
- View information about CD326 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD326 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD326 (Ep-CAM) | W21115A | IHC-F,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-mouse CD326 (Ep-CAM)
IHC staining of purified anti-mouse CD326 (Ep-CAM) (clone W2... IHC staining of purified anti-mouse CD326 (Ep-CAM) (clone W2...
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