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Purified anti-mouse CD36 Antibody

Pricing & Availability
Clone
ZND36-6 (See other available formats)
Regulatory Status
RUO
Other Names
FAT, gpIIIb, gpIV
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
ZND36-6_PURE_CD36_Antibody_1_051221.png
Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with purified anti-mouse CD36 (clone ZND36-6) (filled histogram) or rat IgG2a, κ (open histogram), followed by anti-rat IgG PE.
  • ZND36-6_PURE_CD36_Antibody_1_051221.png
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with purified anti-mouse CD36 (clone ZND36-6) (filled histogram) or rat IgG2a, κ (open histogram), followed by anti-rat IgG PE.
  • ZND36-6_PURE_CD36_Antibody_2_051221.png
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD36 (clone ZND36-6) and anti-mouse CD3ε (clone 500A2) Alexa Fluor® 594 (green) overnight at 4°C, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
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163002 100 µg 106 CHF
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Description

CD36 is a 85 kD glycoprotein, also known as FAT, gpIIIb, or gpIV. It is a member of the class B scavenger receptor family, expressed on platelets, monocytes, macrophages, megakaryocytes, microvasculature, dendritic cells and mammary endothelial cells. The primary ligands for CD36 have been reported to be oxidized low density lipoprotein, anionic phospholipids, and collagens I, IV, and V. CD36 acts as a scavenger receptor thus promoting the removal of apoptotic neutrophils and other apoptotic bodies, as well as clearance of defective erythrocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD36/NIH-3T3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)

1. Tsugita M. et al. 2017. Cell Rep. 18:1298. (FC and IHC-F)

RRID
AB_2892336 (BioLegend Cat. No. 163002)

Antigen Details

Structure
Scavenger receptor superfamily, 85 kD
Distribution

Platelets, monocytes, macrophages, microvascular and mammary endothelial cells

Function
Scavenger receptor, recognition and phagocytosis of apoptotic neutrophils, adhesion
Interaction
Endothelial cells, Macrophages, Monocytes, Platelets
Ligand/Receptor
Oxidized low density lipoprotein, anionic phospholipids, collagen I, IV, and V
Cell Type
Macrophages, Monocytes, Platelets
Biology Area
Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Greenwalt DE, et al. 1992. Blood. 80:1105.
  3. Endemann G, et al. 1993. J Biol Chem. 268:11811.
Gene ID
12491 View all products for this Gene ID
UniProt
View information about CD36 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.12.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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