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Purified anti-mouse CD36L Antibody

Pricing & Availability
Clone
AOB-1 (See other available formats)
Regulatory Status
RUO
Other Names
SCARB1, SR-BI
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
AOB-1_PURE_CD36L_Antibody_1_051321.png
C57BL/6 mouse frozen liver section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD36L (clone AOB-1) overnight at 4°C, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • AOB-1_PURE_CD36L_Antibody_1_051321.png
    C57BL/6 mouse frozen liver section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD36L (clone AOB-1) overnight at 4°C, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • AOB-1_PURE_CD36L_Antibody_2_051321.png
    BALB/c mouse bone-marrow derived macrophages stained with purified anti-mouse CD36L (clone AOB-1) (filled histogram) or rat IgG2a, κ (open histogram) , followed by anti-rat IgG PE.
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163102 100 µg 347 CHF
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Description

Scavenger receptor class B type I (SCARB1), also known as CD36L1, is a member of the scavenger receptor family. It is an integral membrane protein and function as HDL receptor expressed primarily in liver and nonplacental steroidogenic tissues, and mediates selective cholesterol uptake by a mechanism distinct from the classic LDL receptor pathway. Its primary function is facilitating reverse cholesterol transport by transferring cholesterol esters of high-density lipoproteins (HDLs) into the liver. This function serves to lower the risk of development of atherosclerosis. SCARB1 is also able to bind to other lipoproteins such as LDL, OxLDL, and VLDL. SCARB1 is also likely to be involved in pathogen recognition through its recognition of mycobacteria and other viral proteins and regulates vitamin E levels in the tissue.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-F - Quality tested
FC - Verified
IP - Reported in the literature
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)

1. Tsugita M. et al. 2017. Cell Rep. 18:1298.

RRID
AB_2892337 (BioLegend Cat. No. 163102)

Antigen Details

Structure
Integral membrane glycoprotein
Distribution

Liver and nonplacental steroidogenic tissues

Function
Involved in the uptake of high density lipoproteins (HDL), phagocytosis of apoptotic cells, and pathogen recognition.
Ligand/Receptor
Phosphatidylserine, HDL, apoB-containing lipoproteins, LDL, vitamin E
Cell Type
Macrophages, Monocytes, Platelets
Biology Area
Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Acton S, et al. 1996. Science. 5248:518.
  2. Rigotti A, et al. 2003. Endocr Rev. 24:357.
  3. Valacchi G, et al. 2011. Ann N Y Acad Sci. 1229:E1-7.
  4. Scarselli E, et al. 2002. EMBO J. 21:5017.
  5. Mardones P, et al. 2002. J Nutr. 132:443.
Gene ID
20778 View all products for this Gene ID
UniProt
View information about CD36L on UniProt.org
Go To Top Version: 1    Revision Date: 05.13.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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