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Purified anti-mouse CD90.2 (Thy1.2) Antibody

Pricing & Availability
Clone
S20008D (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1, Thy-1.2, CD90, CD90.2, Th1.2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
S20008D_PURE_CD902_Antibody_011122
C57BL/6 mouse splenocytes were pre-treated with TruStain FcX™ PLUS (anti-mouse CD16/32) antibody. Cells were stained with purified anti-mouse CD90.2 (Thy1.2) (clone S20008D) (left) or purified mouse IgG2b, κ isotype control (right), followed by PE anti-mouse IgG2b. Cells were constrained with anti-mouse CD3 APC.
  • S20008D_PURE_CD902_Antibody_011122
    C57BL/6 mouse splenocytes were pre-treated with TruStain FcX™ PLUS (anti-mouse CD16/32) antibody. Cells were stained with purified anti-mouse CD90.2 (Thy1.2) (clone S20008D) (left) or purified mouse IgG2b, κ isotype control (right), followed by PE anti-mouse IgG2b. Cells were constrained with anti-mouse CD3 APC.
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164802 100 µg 147 CHF
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Description

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse Thy1.2 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone S20008D antibody reacts with mouse CD90.2 (Thy 1.2) which is expressed by C57BL/6 and BALB/C strains, but not on CD90.1 strains (AKR/J, PL, FVB, etc.).

Clone S20008D can completely block binding of clone 30-H12 on target cells.

To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody (Cat. No. 156603 & 156604) is recommended prior to staining.

RRID
AB_2910347 (BioLegend Cat. No. 164802)

Antigen Details

Structure
Ig superfamily, 25-35 kD
Distribution

Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice

Function
Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor
CD45
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neurons, T cells, Thymocytes
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Death, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Craig W, et al. 1993. J Exp Med. 177:1331-42.
  3. Reif AE & Schlesinger M. 1989. Cell Surface Antigen Thy-1.
  4. Mayani H, et al. 1994. Blood. 83:2410-7.

 

Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90.2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12.28.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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