Purified anti-mouse Granzyme C

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Clone: SFC1D8 (See other available formats)
Regulatory Status: RUO
Other Names: CCP2, Ctla5, Ctla-5, AI323531, B10, GzmC
Isotype: Armenian Hamster IgG
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Product Citations: publications

SFC1D8_PURE_Granzyme_C_Antibody_1_022217 — CD3+CD28 stimulated BALB/c splenocytes (in the presence of recombinant mouse IL-2) were stained with CD8 APC and purified anti-mouse Granzyme C (clone SFC1D8, top) or purified Armenian Hamster IgG isotype control (bottom), followed by anti-hamster IgG FITC.

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150802

500 µg

317 CHF

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Description

Granzyme C is a serine protease that is found in the granules of cytotoxic T lymphocytes and natural killer cells (NKs). It induces the apoptosis of target cells by the externalization of phosphatidylserine, nuclear condensation and collapse, as well as the introduction of single-stranded DNA nicks. It can also induce apoptosis via mitochondrial swelling, which results in the loss of membrane potential. Reported studies show that granzyme C can support CTL-mediated killing in the absence of granzyme A or B. Additionally, wild-type mice have delayed upregulation of granzyme C relative to granzyme B and a mutation in the granzyme B gene shows earlier upregulation of granzyme C. Further studies show that in the event that granzyme B fails, the mouse models infected with persistent viruses have delayed expression of protection provided by granzyme C.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Purified recombinant granzyme C in CFA and boosted in IFA.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2650698 (BioLegend Cat. No. 150802)

Antigen Details

Distribution: Cytotoxic T lymphocytes and natural killer (NK) cells.
Function: Induces apoptotic cell death.
Cell Type: NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Enzymes and Regulators, Proteases
Antigen References: 1. Bots M, et al. 2006. J. Cell. Sci. 119:5011.
2. Ley TJ, et al. 2003. Blood 101:3093.
3. Revell PA. 2005. J. Immunol. 2005. 174:2124.
4. Kelso A. 2002. Int. Immunol. 6:605.
5. Getachew Y, et al. 2008. J. Immunol. 181:7810.
6. Cai Sheng, et al. 2009. J. Immunol. 182:6287.
Gene ID: 14940 View all products for this Gene ID
UniProt: View information about Granzyme C on UniProt.org

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View All Granzyme C Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-mouse Granzyme C	SFC1D8	ICFC
Purified anti-mouse Granzyme C	SFC1D8	ICFC
FITC anti-mouse Granzyme C	SFC1D8	ICFC
PE/Cyanine7 anti-mouse Granzyme C	SFC1D8	ICFC
PerCP/Cyanine5.5 anti-mouse Granzyme C	SFC1D8	ICFC
APC anti-mouse Granzyme C	SFC1D8	ICFC

Certificate of Analysis

Go To Top Version: 0 Revision Date: 03.14.2017

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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