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Purified anti-mouse H-2Kb/H-2Db Antibody

Pricing & Availability
Clone
28-8-6 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class I
Isotype
Mouse (C3H) IgG2a, κ
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Product Citations
publications
28-8-6_Purified_H-2KH-2D_Antibody_FC_050713
C57BL/6 mouse splenocytes were stained with purified H-2Kb/H-2Db (clone 28-8-6) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG2a PE.
  • 28-8-6_Purified_H-2KH-2D_Antibody_FC_050713
    C57BL/6 mouse splenocytes were stained with purified H-2Kb/H-2Db (clone 28-8-6) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG2a PE.
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114602 500 µg 256 CHF
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Description

The 28-8-6 antibody reacts with the H-2Kb and H-2Db MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kb/H-2Db haplotype. H-2Kb/H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-8-6 antibody cross-reacts with H-2Dd MHC class I alloantigen, but does not react with alloantigens of f, k, p, q, r, s haplotypes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C3H.SW mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ozato K, et al. 1981. J. Immunol. 126:317. (Cyt)
  2. Pappo J, et al. 1999. Infect. Immun. 67:337. (IHC)
  3. Bui JD, et al.2006. J. Immunol. 176:905. (FC) PubMed.
  4. Shao H, et al. 2005. J. Immunol. 175:1851. (FC)
Product Citations
  1. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  2. Woodham AW, et al. 2018. Cancer Immunol Res. 0.854166667. PubMed
  3. Wei SC, et al. 2019. Immunity. 50:1084. PubMed
  4. Sun Y, et al. 2022. Nat Commun. 13:3916. PubMed
  5. Zhu XG, et al. 2020. Cell Metabolism. 33(1):211-221.e6. PubMed
  6. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  7. Bui J, et al. 2006. J Immunol. 176:905. PubMed
RRID
AB_313593 (BioLegend Cat. No. 114602)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Kb/H-2Db haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Ozato K, et al. 1981. J. Immunol. 126:317.
2. Allen H, et al. 1986. P. Natl. Acad. Sci. USA 83:7447.
3. Evans GA, et al. 1992. Nature 300:755.

Gene ID
14964 View all products for this Gene ID 14972 View all products for this Gene ID
UniProt
View information about H-2Kb/H-2Db on UniProt.org
Go To Top Version: 3    Revision Date: 08.16.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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