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Purified anti-mouse/human Ki-67 Antibody

Pricing & Availability
Clone
11F6 (See other available formats)
Regulatory Status
RUO
Other Names
Mki67, Ki67, Ki-67, MIB-1, KIA
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
11F6_PURE_Ki-67_1_010616edit
TE-71 cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml Ki-67 (clone 11F6) in 5% FBS overnight at 4°C, followed by Alexa Fluor® 647 Goat anti-rat IgG (clone Poly4054) for two hours and Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • 11F6_PURE_Ki-67_1_010616edit
    TE-71 cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml Ki-67 (clone 11F6) in 5% FBS overnight at 4°C, followed by Alexa Fluor® 647 Goat anti-rat IgG (clone Poly4054) for two hours and Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • 11F6_PURE_Ki-67_2_010616edit
    C57BL/6 mouse frozen intestine section was fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS plus 5% goat serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml Ki-67 (clone 11F6) purified in 5% FBS overnight at 4°C, followed by 2.5 µg/mL of goat anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) and 5 µg/ml of E-cadherin (clon
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151202 100 µg 136 CHF
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Description

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase. While in G0 phase, the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-F - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/ml is recommended. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 1.25 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Su T, et al. 2021. Metabolites. 11:. PubMed
  2. Miyauchi K, et al. 2021. Front Immunol. 12:663177. PubMed
  3. Cai S, et al. 2020. Sci Rep. 10:14249. PubMed
  4. Aghayev T, et al. 2022. Cancer Discov. 12:1960. PubMed
  5. Flores–Santibáñez F, et al. 2018. Front Immunol. 0.520138889. PubMed
  6. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  7. Schrage R, et al. 2015. Nat Commun. 6: 10156. PubMed
  8. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  9. Reyes RV, et al. 2022. Front Neurosci. 16:908719. PubMed
  10. Coover RA, et al. 2018. Acta Neuropathol Commun. 6:127. PubMed
  11. Viereckl MJ, et al. 2022. Biomolecules. 12:. PubMed
  12. Lee YS, et al. 2018. Cell Host Microbe. 24:833. PubMed
  13. Ren J, et al. 2018. Cell Cycle. 1.25. PubMed
RRID
AB_2566621 (BioLegend Cat. No. 151202)

Antigen Details

Structure
325 kD protein containing a forkhead-associated (FHA) domain and 13 tandem repeats.
Distribution

Nucleus and chromosomes.

Function
Required for cell cycle progression and proliferation.
Biology Area
Cell Biology, Cell Cycle/DNA Replication
Antigen References
  1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
  2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
  3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
  4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
  5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
  6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID
4288 View all products for this Gene ID 17345 View all products for this Gene ID
UniProt
View information about Ki-67 on UniProt.org
Go To Top Version: 2    Revision Date: 01.14.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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