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Purified anti-mouse/human SEMA4A Antibody

Pricing & Availability
Clone
5E3/SEMA4A (See other available formats)
Regulatory Status
RUO
Other Names
Semaphorin 4A, SEMAB, Sema B, Semaphorin B, RP35, CORD10, SEMA 4A
Isotype
Mouse IgG1, λ
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Product Citations
publications
5E3-SEMA4A_Purified_SEMA4A_Antibody_FC_1_072914.jpg
Human peripheral blood monocyte derived dendritic cells were stained with purified Semaphorin 4A (SEMA4A) (clone 5E3/SEMA4A) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.
  • 5E3-SEMA4A_Purified_SEMA4A_Antibody_FC_1_072914.jpg
    Human peripheral blood monocyte derived dendritic cells were stained with purified Semaphorin 4A (SEMA4A) (clone 5E3/SEMA4A) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.
  • 5E3-SEMA4A_Purified_SEMA4A_Antibody_FC_2_072914.jpg
    C57BL/6 mouse splenocytes were stained with CD3 APC and purified Semaphorin 4A (SEMA4A) (clone 5E3/SEMA4A) (above) or purified mouse IgG1, κ isotype control (below), followed by anti-mouse IgG1 PE.
  • 5E3-SEMA4A_Purified_SEMA4A_Antibody_FC_3_072914.jpg
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148402 100 µg 276 CHF
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Description

Semaphorin 4A (SEMA4A) is a class IV semaphorin of soluble and transmembrane proteins originally identified as SemB. It is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain, and a Sema domain. SEMA4A is expressed on dendritic cells, activated B cells, and T cells. It is also found on different tissues, such as those in brain, lung, kindney, spleen, and testis. In the immune system, SEMA4A functions as an activator in the T-cell-mediated immune response and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Like other semaphorin family members it is also involved in guidance of axonal migration during neuronal development and in immune responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse SEMA4A-Fc proteins
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include: ELISA1.

Application References

(PubMed link indicates BioLegend citation)
  1. Kumanogoh A. et al. 2005. Immunity. 22:305. (ELISA)
RRID
AB_2564113 (BioLegend Cat. No. 148402)

Antigen Details

Structure
Single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a SEMA domain.
Distribution

Dendritic cells, T cells, B cells, brain, lung, kindney, spleen and testis

Function
An activator in T-cell-mediated immune response. Suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. It is also involved in guidance of axonal migration during neuronal development.
Cell Type
Dendritic cells, T cells, B cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules
Antigen References

1. Kumanogoh A, et al. 2002. Nature. 419:629. 
2. Kumanogoh A, et al. 2005. Immunity. 22:305. 
3. Toyofuku T, et al. 2007. EMBO J. 26:1373. 
4. Delgoffe GM, et al. 2013. Nature. 501:252.

Gene ID
20351 View all products for this Gene ID 64218 View all products for this Gene ID
UniProt
View information about SEMA4A on UniProt.org
Go To Top Version: 2    Revision Date: 11.13.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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