Purified anti-mouse/human Siglec-15 Antibody

Pricing & Availability
Clone
MK4.5 (See other available formats)
Regulatory Status
RUO
Other Names
Sialic acid-binding Ig-like lectin 15, CD33 antigen-like 3
Isotype
Mouse IgG1, κ
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Product Citations
publications
MK4.5_PURE_Siglec-15_Antibody_032822_updated
Raw 264.7 cells were stimulated with human TRANCE for 4 days (filled histogram) or were cultured without stimulus (open histogram) and were then stained with purified anti-mouse/human Siglec-15 (clone MK4.5), followed by PE anti-mouse IgG1.
  • MK4.5_PURE_Siglec-15_Antibody_032822_updated
    Raw 264.7 cells were stimulated with human TRANCE for 4 days (filled histogram) or were cultured without stimulus (open histogram) and were then stained with purified anti-mouse/human Siglec-15 (clone MK4.5), followed by PE anti-mouse IgG1.
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164602 100 µg 276 CHF
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Description

Siglec-15, also known as Siglec-I and CD33L3, is a member of the Sialic acid-binding Ig-like lectin family and is a type I single pass transmembrane protein. Siglec-15 is expressed by a subset of myeloid cells, osteoclasts, and can be upregulated by certain cancers. Siglec-15 plays an important role in osteoclastogenesis and bone remodeling during normal development and in bone absorption in arthritis. Furthermore, Siglec-15 suppresses antigen-specific T cell responses and is therefore considered to be an immune suppressor and potential target for normalization cancer immunotherapy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse Siglec-15-huIgG Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, ICFC, Direct ELISA, ELISA Capture - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Korn M, et al. 2020. J Immunol. 205:2595-2605.
RRID
AB_2922502 (BioLegend Cat. No. 164602)

Antigen Details

Structure
Type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain containing a lysine residue, and a short cytoplasmic tail
Distribution

Myeloid lineage, osteoclasts, and upregulated by certain types of human cancers

Function
Regulates osteoclast development and bone resorption
Interaction
DAP12
Ligand/Receptor
Neu5Ac alpha2-6GalNAc alpha
Cell Type
Macrophages, Osteoclasts
Biology Area
Immunology
Molecular Family
Siglec Molecules
Antigen References
  1. Wang J, et al. 2019. Nat Med. 25:656-666.
  2. Angata T. 2020. J Biomed Sci. 27:10.
  3. Takamiya R, et al. 2013. Glycobiology. 23:178-87.
Gene ID
620235 View all products for this Gene ID 284266 View all products for this Gene ID
UniProt
View information about Siglec-15 on UniProt.org

Other Formats

View All Siglec-15 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse/human Siglec-15 MK4.5 FC,ICFC,ICC,ELISA Capture,Direct ELISA
Go To Top Version: 1    Revision Date: 03.01.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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