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Purified anti-mouse I-Ad Antibody

Pricing & Availability
Clone
39-10-8 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Mouse (C3H.SW) IgG3, κ
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Product Citations
publications
39-10-8_PURE_I-A_Antibody_091117
BALB/c mouse splenocytes were stained with purified anti-mouse I-Ad (clone 39-10-8, filled histogram) or purified mouse IgG3, κ isotype control (open histogram), followed by anti-mouse IgG3 PE.
  • 39-10-8_PURE_I-A_Antibody_091117
    BALB/c mouse splenocytes were stained with purified anti-mouse I-Ad (clone 39-10-8, filled histogram) or purified mouse IgG3, κ isotype control (open histogram), followed by anti-mouse IgG3 PE.
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115002 500 µg 253 CHF
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Description

The 39-10-8 antibody reacts with the I-Ad MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2d bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 39-10-8 antibody does not cross-react with other haplotypes (a, b, k, p, q, s), but has been demonstrated to cross-react with the g7 haplotype.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
(C3H x BALB/c)F1 mouse cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Application

FC - Quality tested
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, and immunohistochemical staining of acetone-fixed frozen sections.

Does not cross-react with other haplotypes (e.g., a, b, k, p, q, s).

Application References

(PubMed link indicates BioLegend citation)
  1. Hiramine C, et al. 1995. Cell. Immunol. 160:157.
  2. Wang Z, et al. 2004. J. Immunol. 172:5924.
  3. Ma XT, et al. 2006. Cancer Research 66:1169.
  4. Norian LA and Allen PM. 2004. J. Immunol. 173:835. PubMed
  5. Tian C, et al. 2007. J. Immunol. 179:6762.
Product Citations
  1. Ebrahimi-Nik H, et al. 2021. Nat Commun. 12:6423. PubMed
  2. Allen L 2004. J Immunol. 173:835. PubMed
RRID
AB_313617 (BioLegend Cat. No. 115002)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of H-2d mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. Wall KA, et al. 1983. J. Immunol. 131:1056.
4. Ridgway WM, et al. 1998. J. Exp. Med. 188:2267.

Gene ID
14961 View all products for this Gene ID
UniProt
View information about I-Ad on UniProt.org
Go To Top Version: 3    Revision Date: 08.18.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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