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Purified anti-mouse IFN-β Antibody

Pricing & Availability
Clone
MIB-5E9.1 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-β, Fibroblast interferon (Fi-IFN, F-IFN), Type-1 interferon, acid-stable interferon, R1-G1 factor, IFN-b, IFN-beta
Isotype
Armenian Hamster IgG
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Product Citations
publications
mIFN-b_MIB-5E9.1_081611
Cell extracts of 293E cells transfected with mouse IFN-β-1 were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-mouse IFN-β-1 antibody (clone MIB-5E9.1). Proteins were visualized using a goat anti-Armenian Hamster IgG secondary conjugated to HRP and chemiluminescence detection.
  • mIFN-b_MIB-5E9.1_081611
    Cell extracts of 293E cells transfected with mouse IFN-β-1 were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-mouse IFN-β-1 antibody (clone MIB-5E9.1). Proteins were visualized using a goat anti-Armenian Hamster IgG secondary conjugated to HRP and chemiluminescence detection.
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508102 500 µg 282 CHF
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Description

Interferon-β is a member of the type I interferon class. Type I IFNs are involved in the innate immune system, being released non-specifically during the early stage of infection in response to stimuli such as viruses, dsRNA, LPS, and bacterial components. Once secreted by virus-infected or activated cells, they bind to the IFNα/β receptor (IFNAR), which is found on almost all cell types, and, via the JAK-STAT pathway, induce the expression of hundreds of IFN-stimulated genes (ISGs). While the function of many of these proteins remains to be elucidated, a hallmark of type I IFNs is their anti-viral activities, both general and specific against particular viruses. Type I IFNs also regulate cell growth and apoptosis, and exert various immunomodulatory and inflammatory effects. As such, they are also clinically relevant, both as therapeutics and as targets of inhibition for the treatment of various autoimmune diseases and cancer.

Mouse IFN-β is a ~20 kD monomeric glycoprotein. It has 50% amino acid sequence homology with mouse IFN-α, its closest related protein, and 62% homology with human IFN-β. IFN-β is currently the therapeutic of choice for treating multiple sclerosis.

 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Neut - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. The suggested optimal usage is 1 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: To measure mouse IFN-ß, the biotinylated MIB-5E9.1 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified Poly5192 antibody (Cat. No. 519202) as the capture antibody and Recombinant Mouse IFN-ß (ELISA Std.) (Cat. No. 581309) as the standard.
Western Blotting: The purified antibody is recommended for Western blotting (Cat. Nos. 508102 & 508104).
Neutralization1: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-ß bioactivity (Cat. Nos. 508107 & 508108).
Note: For testing mouse IFN-ß in serum, plasma or cell culture supernatant, BioLegend's ELISA LEGEND MAX™ Kits (Cat. Nos. 439407 & 439408) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Creasy BM and McCoy KL. 2011. Cell Immunol. 267:56. (Neut) PubMed
  2. Sow FB, et al. 2009. J. Leukoc. Biol. 86:1247. PubMed
  3. Zaric SS, et al. 2011. J. Biol Chem. 286:29492. PubMed
Product Citations
  1. Joshi JC, et al. 2020. Cell Rep. 30:4096. PubMed
RRID
AB_315505 (BioLegend Cat. No. 508102)

Antigen Details

Structure
Interferon; 26-35 kD (Mammalian)
Distribution

Fibroblasts, epithelial cells

Ligand/Receptor
IFNα/βR (IFNAR-1, IFNAR-2)
Bioactivity
Non-specific humoral immune responses and immune responses against viral infections, increases expression of MHC class I antigens, blocks expression of MHC class II antigens stimulated by IFN-γ
Cell Targets
Broad, includes NK cells, T suppressor cells, activated monocytes
Cell Type
Epithelial cells, Fibroblasts
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Nagarajan U. 2011. Crit. Rev. Immunol. 31:459.
2. Honda K, et al. 2005. Int. Immunol. 17:1367.

Regulation
Upregulated by viruses, double-stranded RNA, micro-organisms, TNF, IL-1
Gene ID
15977 View all products for this Gene ID
UniProt
View information about IFN-beta on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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