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Purified anti-mouse IL-12/IL-23 p40 (monomer, dimer, heterodimer) Antibody

Pricing & Availability
Clone
C15.6 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)
Isotype
Rat IgG1, κ
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Product Citations
publications
MouseIL-12p70_061604
  • MouseIL-12p70_061604
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505201 50 µg 83 CHF
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505202 500 µg 235 CHF
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Description

The C15.6 antibody reacts with mouse IL-12 p40 subunit of the IL-12 p70 and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer, or heterodimer. The C15.6 antibody can not neutralize the bioactivity of natural or recombinant IL-12.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO-expressed, recombinant mouse IL-12 p70
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
IHC-F, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1.0 - 4.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-12 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture1,2,4,6,8,10: The purified C15.6 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated C17.8 (Cat. No. 505302) antibody as the detecting antibody. This assay detects p40 as monomer, homodimer, or heterodimer complexed with p35. The LEAF™ purified antibody is suggested for ELISPOT capture.
Additional reported applications (for the relevant formats) include: immunohistochemical staining9 of paraformaldehyde-fixed saponin-treated frozen tissue sections, immunoprecipitation3, and Western blotting3.
Note: For testing mouse IL-12 p40 (monomer, dimer, heteromer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431601 to 431606) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260.
  2. Reichmann G, et al. 1999. J. Immunol. 163:3354.
  3. Wysocka M, et al. 1995. Eur. J. Immunol. 25:672.
  4. Gately M. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.16.
  5. Macatonia S, et al. 1995. J. Immunol. 154:5071.
  6. O'Connell PJ, et al. 2006. Blood 107:1010.
  7. Akilov OE, et al. 2007. J. Leukocyte Biol. 2007;10.1189/jlb.0706439.
  8. Dzhagalov I, et al. 2007. J. Immunol. 178:2113.
  9. Huang LY, et al. 2001. J. Immunol. 167:1423.
  10. Xu G, et al. 2007. J. Immunol. 179:5358. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. Malu DT, et al. 2011. J. Immunol. 186:6271. PubMed
Product Citations
  1. Rosas Almanza J, et al. 2022. J Neurosci Res. 100:2213. PubMed
  2. Adhikari AS, et al. 2022. Front Oncol. 12:850546. PubMed
  3. Li H, et al. 2018. Nat Commun. 9:2830. PubMed
  4. Johnson SA, et al. 2021. Eur J Immunol. 51:3228. PubMed
  5. Ranjan K, et al. 2021. J Clin Invest. 131:. PubMed
  6. Xu G, et al. 2007. J Immunol. 179:5358. PubMed
  7. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  8. Malu D, et al. 2011. J Immunol. 186:6271. PubMed
RRID
AB_315366 (BioLegend Cat. No. 505201)
AB_315366 (BioLegend Cat. No. 505202)

Antigen Details

Structure
Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
Bioactivity
IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human me
Cell Sources
Dendritic cells, monocytes/macrophages, B cells, T cells
Cell Targets
T cells, NK cells
Receptors
IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Oppmann B, et al. 2000 Immunity. 13:715.
6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910.
7. Parham C, et al. 2002 J. Immunol. 168:5699.
8. Belladonna ML, et al. 2002 J. Immunol. 168:5448.
9. Lankford, CS, et al. 2003 J. Leukocyte Biol. 73:49.

Regulation
Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
Gene ID
16160 View all products for this Gene ID
UniProt
View information about IL-12/IL-23 p40 on UniProt.org

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Go To Top Version: 4    Revision Date: 09.20.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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