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Purified anti-mouse IL-15 Antibody

Pricing & Availability
Clone
W19387G (See other available formats)
Regulatory Status
RUO
Other Names
IL-15
Isotype
Rat IgG2a, κ
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W19387G_PURE_IL-15_Antibody_022024
Direct ELISA showing purified anti-mouse IL-15 (clone W19387G) bound to plate-immobilized recombinant mouse IL-15 (Cat. Nos. 566301, 566302, 566304). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-rat IgG (Cat. No. 405405). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • W19387G_PURE_IL-15_Antibody_022024
    Direct ELISA showing purified anti-mouse IL-15 (clone W19387G) bound to plate-immobilized recombinant mouse IL-15 (Cat. Nos. 566301, 566302, 566304). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-rat IgG (Cat. No. 405405). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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631552 100 µg 420 CHF
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Description

IL-15 was discovered in the supernatant of Simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line CTLL-2. IL-15 is a regulatory cytokine, and it is produced by dendritic cells, epithelial cells, fibroblasts, and monocytes. IL-15 plays an important role in immune response and shares many functions with IL-2. For example, it stimulates the proliferation of activated T cells, NK cells, and B cells, and induces immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells and induces the production of proinflammatory cytokines from macrophages. IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system. IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL-15Rβ, and IL-15Rγc. IL-15 shares the receptor chains β and γc with IL-2. IL-15 is normally not secreted in soluble form but is instead held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. Cell-bound IL-15 is then presented in trans form to T cells and NK cells and is recognized by the γc receptor on these cells; such recognition maintains cell survival and intermittent proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Recombinant IL-15 Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Antigen Details

Structure
Cytokine
Distribution

IL-15 is expressed by DC, epithelial cells, fibroblasts, and monocytes.

Function
IL-15 stimulates the proliferation of activated T cells, NK cells, and B cells, and inducing immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells, and induces the production of proinflammatory cytokines from macrophages.
Ligand/Receptor
IL-15Rα, IL-15Rβ and IL-15γc
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Grabstein K, et al. 1994. Science. 264:965-8.
  2. Ma A, et al. 2006. Annu Rev Immunol. 24:657-79.
  3. Meresse B, et al. 2004. Immunity. 21:357-66.
  4. Armitage RJ, et al. 1995. J Immunol. 154:483-90.
  5. Pulendran B, et al. 2004. Eur J Immunol. 34:66-73.
  6. Bouchard A, et al. 2004. J Leukoc Biol. 75:893-900.
  7. Ratthe C, et al. 2004. J Leukoc Biol. 75:893-900.
  8. Feng T, et al. 2008. Cell Immunol. 5:189.
  9. Rubinstein MP, et al. 2006. P Natl Acad Sci USA. 103:9166.
Gene ID
16168 View all products for this Gene ID
UniProt
View information about IL-15 on UniProt.org
Go To Top Version: 1    Revision Date: 02.22.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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