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Purified anti-mouse IL-2 Antibody

Pricing & Availability
Clone
JES6-1A12 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
Isotype
Rat IgG2a, κ
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Product Citations
publications
IL-2 Mouse
  • IL-2 Mouse
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503701 50 µg 83 CHF
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503702 500 µg 143 CHF
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Description

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes. The JES6-1A12 antibody reacts with mouse interleukin-2 (IL-2).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse IL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-2 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture1-3 or ELISPOT Capture4-6: The purified JES6-1A12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES6-5H4 antibody (Cat. No. 503804) as the detecting antibody and recombinant mouse IL-2 (Cat. No.575409) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 503704).
Neutralization: The JES6-1A12 antibody can neutralize the bioactivity of natural or recombinant IL-2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 503706) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  2. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
  3. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
  4. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19.
  5. Mo X, et al. 1995. J. Virol. 69:1288.
  6. Karulin A, et al. 2000. J. Immunol. 164:1862.
  7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28.
Product Citations
  1. Sato A, et al. 2022. Biol Pharm Bull. 45:1798. PubMed
  2. Galaski J, et al. 2021. Front Cell Infect Microbiol. 11:692544. PubMed
  3. Nishio J, et al. 2010. J Exp Med. 207:1879. PubMed
  4. Secco V, et al. 2008. Biol Reprod. 78:234. PubMed
  5. Lin YJ, et al. 2021. Cells. 10:. PubMed
  6. Parrish H, et al. 2015. PLoS One. 10: 0132333. PubMed
  7. Newland SA, et al. 2017. Nat Commun. 8:15781. PubMed
  8. Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed
  9. Phan TS, et al. 2021. Sci Adv. 7:. PubMed
  10. Devine L, et al. 2006. J Immunol. 177:3930. PubMed
RRID
AB_315291 (BioLegend Cat. No. 503701)
AB_315291 (BioLegend Cat. No. 503702)

Antigen Details

Structure
Cytokine; 15-30 kD (Mammalian)
Bioactivity
Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Cell Sources
T cells
Cell Targets
T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors
High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.

Regulation
Upregulated by NFAT; downregulated by TCF-8, CIF (colostrum inhibitory factor)
Gene ID
16183 View all products for this Gene ID
UniProt
View information about IL-2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All IL-2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse IL-2 JES6-1A12 ELISA Capture,IP,WB
Ultra-LEAF™ Purified anti-mouse IL-2 JES6-1A12 ELISA Capture,ELISPOT Capture,IP,Neut,WB
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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