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Purified anti-mouse IL-3 Antibody

Pricing & Availability
Clone
MP2-8F8 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor
Isotype
Rat IgG1, κ
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Product Citations
publications
Mouse_IL-3_TMB_020305
  • Mouse_IL-3_TMB_020305
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503901 50 µg 106 CHF
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503902 500 µg 138 CHF
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Description

IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The MP2-8F8 antibody reacts with mouse interleukin-3 (IL-3). The MP2-8F8 antibody can neutralize the bioactivity of natural or recombinant IL-3.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-expressed, recombinant mouse IL-3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
IP, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-3 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture1-5,8: The purified MP2-8F8 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP2-43D11 antibody (Cat. No. 504002) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams, J., et al. 1992. Immunol. Rev. 127:5.
  2. Abrams, J., et al. 1988. J. Immunol. 140:131.
  3. Cockayne, D., et al. 1991. Growth Factors 5:171.
  4. Sander, B., et al. 1993. J. Immunol. Meth. 166:201.
  5. Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
  6. Finkelman, F., et al. 1993. J. Immunol. 151:1235.
  7. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  8. Karulin, A., et al. 2000. J. Immunol. 164:1862.
Product Citations
  1. Fujita H, et al. 2022. Cells. 11:. PubMed
  2. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
RRID
AB_315307 (BioLegend Cat. No. 503901)
AB_315307 (BioLegend Cat. No. 503902)

Antigen Details

Structure
Cytokine; 28 kD (Mammalian)
Bioactivity
Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase
Cell Sources
Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells
Cell Targets
Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages
Receptors
Heterodimer IL-3Rα (CD123) and two alternate β-subunits, AIC2A binds IL-3 and is specific to IL-3Rα, AIC2B (CDw131) shared in common with IL-5R, GM-CSFR
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Frendl, G., 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle, J., 1992. Chem. Immunology 51:65.
4. Valen T, P., et al. 1990. Blut 61:338.

Regulation
Upregulated by T cell stimulation; downregulated by glucocorticoids
Gene ID
16187 View all products for this Gene ID
UniProt
View information about IL-3 on UniProt.org

Other Formats

View All IL-3 Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse IL-3 MP2-8F8 ICFC
Purified anti-mouse IL-3 MP2-8F8 ELISA Capture,IHC,IP
APC anti-mouse IL-3 MP2-8F8 ICFC
Ultra-LEAF™ Purified anti-mouse IL-3 MP2-8F8 ELISA Capture,ELISPOT Capture,Neut
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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