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Purified anti-mouse IL-33 Antibody

Pricing & Availability
Clone
Poly5165 (See other available formats)
Regulatory Status
RUO
Other Names
Il-1f11, Nuclear factor from high endothelial venules (NF-HEV)
Isotype
Goat Polyclonal Ig
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Product Citations
publications
Poly5165_Mouse_IL-33_102512
  • Poly5165_Mouse_IL-33_102512
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516501 100 µg 350 CHF
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Description

IL-33 belongs to the IL-1 family and is closely related in structure to IL-18 and IL-1b. IL-33, IL-1b, and IL-18 are synthesized as biologically inactive precursor and are cleaved by the enzyme caspase-1 to be secreted as active mature forms. IL-33 stimulates target cells by binding to the IL-1R/TLR superfamily member ST2 and subsequently activates NF-κB and MAPK pathways via identical signalling events to those observed for IL-1b. In addition, IL-33 is a nuclear factor (NF-HEV) abundantly expressed in high endothelial venules from lymphoid organs that associates with chromatin and exhibits transcriptional regulatory properties.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Mouse IL-33, amino acids Ser109-Ile266 (Accession# NM_133775) was expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by antigen affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 0.5-2 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-33 recombinant protein ranging from 1000-15.6 pg/ml are recommended.

Application Notes

ELISA Capture: The purified Poly5165 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5165 antibody (Cat. No. 516503) as the detecting antibody and recombinant mouse IL-33 (Cat. No. 580509) as the standard.

 

Application References

(PubMed link indicates BioLegend citation)
  1. Gao X, et al. 2015. J Immunol. 194:438. PubMed
Product Citations
  1. Gao X, et al. 2015. J Immunol. 194:438. PubMed
RRID
AB_2561333 (BioLegend Cat. No. 516501)

Antigen Details

Distribution

High levels of mouse IL-33 mRNA are detected in the stomach, lung, spinal cord, brain, and skin. Expression within these organs is restricted to a few cell types, such as epithelial cells, fibroblasts, and smooth muscle cells. In addition, mouse IL-33 mRNA is found in resting dendritic cells and activated macrophages. Endothelial cells constitute a major source of IL-33 mRNA in chronically inflamed tissues.

Function
IL-33 drives production of Th2-associated cytokines from in vitro polarized Th2 cells. In mice, IL-33 injection induced the expression of IL-4, IL-5, and IL-13 and led to severe pathological changes in the lung and the digestive tract. In addition, IL-33 acts as a chemoattractant for Th2 cells, both in vitro and in vivo. TNF-a and IL-1b are activators of IL-33 transcription in fibroblasts and keratinocytes.
Ligand/Receptor
IL-33 binds to the IL-1 family receptor T1/ST2 and IL-1RAcP (IL-1 receptor associated protein).
Cell Type
Epithelial cells, Fibroblasts
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Schmitz J, et al. Immunity 2005 23:479
2. Barksby HE, et al. C Exp Immunol 2007 149:217
3. Arend WP, et al. ImmRev 2008 223:20
4. Suzukawa M, et al. J. Immunol. 2008 181:5981
5. Moussio C, et al. PlosOne 2008 10:e3331.

Gene ID
77125 View all products for this Gene ID
UniProt
View information about IL-33 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All IL-33 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse IL-33 Poly5165 ELISA Detection
Purified anti-mouse IL-33 Poly5165 ELISA Capture
Go To Top Version: 2    Revision Date: 12.16.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-mouse IL-33

    Poly5165_Mouse_IL-33_102512

  • Purified anti-mouse IL-33

    Poly5165_Mouse_IL-33_102512
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