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Purified anti-mouse IL-33Rα (IL1RL1, ST2) Antibody

Pricing & Availability
Clone
W17243F (See other available formats)
Regulatory Status
RUO
Other Names
IL-33R, ST2, IL-1RL1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17243F_PURE_IL-33Ra_Antibody_1_051122
Mouse splenocytes were stained with anti-mouse CD4 APC/Cyanine7, anti-mouse FOXP3 Brilliant Violet 421™ and purified anti-mouse IL-33Rα (IL1RL1, ST2) (clone W17243F) (left) or purified rat IgG2a, κ isotype control (right) followed by PE anti-rat IgG. Data shown was gated on CD4-positive cells.
  • W17243F_PURE_IL-33Ra_Antibody_1_051122
    Mouse splenocytes were stained with anti-mouse CD4 APC/Cyanine7, anti-mouse FOXP3 Brilliant Violet 421™ and purified anti-mouse IL-33Rα (IL1RL1, ST2) (clone W17243F) (left) or purified rat IgG2a, κ isotype control (right) followed by PE anti-rat IgG. Data shown was gated on CD4-positive cells.
  • W17243F_PURE_IL-33Ra_Antibody_2_051122
    ST2-FLAG transfected DO-11.10 mouse cells were stained with purified anti-mouse IL-33Rα (IL1RL1, ST2) (clone W17243F) (filled histogram), or purified rat IgG2a, κ isotype control (open histogram) followed by PE anti-rat IgG.
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165302 100 µg 258 CHF
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Description

IL-33Rα, also known as ST2 or IL-1RL1, is a member of the Toll/IL-1 receptor family. It binds IL-33 and is structurally similar to IL-1R1. Two forms of the protein exist: a soluble form known as ST2 and a membrane anchored form known as ST2L. The membrane form is expressed by Th2 cells and bone marrow derived mast cells, whereas the soluble form is expressed by serum-stimulated fibroblasts. Signaling through IL1RL1 drives cytokine production in mast cells, basophils, eosinophils, invariant NKT and NK cells, Th2 lymphocytes, and type 2 innate immune cells. IL-33 signaling is required for maintaining the suppressive function of regulatory T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse IL-33Rα, AA 27-342
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2922503 (BioLegend Cat. No. 165302)

Antigen Details

Structure
Single-pass type I membrane protein
Distribution

Th2 lymphocytes, mast cells, fibroblasts

Function
Following interaction with IL-33, it recruits MyD88, Irak1, Irak4 and Traf6 followed by phosphorylation of the MAP/ERK pathway
Interaction
Interacts with MyD88, Irak1, Irak4 and Traf6
Ligand/Receptor
IL-33
Cell Type
Fibroblasts, Mast cells, Th2
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokine/Chemokine Receptors
Antigen References
  1. Yanagisawa K, et al. 1993. FEBS Lett. 318:83-7.
  2. Schmitt E, et al. 1990. Cytokine. 2:407-15.
  3. Yanagisawa K, et al. 1992. FEBS Lett. 302:51-3.
  4. Takagi T, et al. 1993. Biochim Biophys Acta. 1178:194-200.
Gene ID
9173 View all products for this Gene ID
UniProt
View information about IL-33Ralpha on UniProt.org
Go To Top Version: 1    Revision Date: 05.05.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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