Purified anti-mouse Ly-6C Maxpar Ready Antibody anti-Ly-6C

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Clone: HK1.4 (See other available formats)
Regulatory Status: RUO
Other Names: Lymphocyte antigen 6 complex, locus C
Isotype: Rat IgG2c, κ
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Product Citations: publications

HK1.4_Purified_Ly-6C_Cytof_061014.jpg — Mouse splenocytes stained with 150Nd-anti-Ly-6C (HK1.4) and 146Nd-anti-CD8 (53-6.7). T lymphocytes are displayed in the analysis. Data provided by DVS Sciences.

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128039

100 µg

124 CHF

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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8⁺ T cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: L3 cloned CTL cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Application Notes: Clone HK1.4 does not block the binding of clone RB6-8C5⁸.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells^1-3 and immunohistochemistry of frozen sections⁴.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2563783 (BioLegend Cat. No. 128039)

Antigen Details

Structure: 14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution: Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8⁺ T cells.
Cell Type: Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.
Gene ID: 17067 View all products for this Gene ID
UniProt: View information about Ly-6C on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All Ly-6C Reagents Request Custom Conjugation

Description	Clone	Applications
Pacific Blue™ anti-mouse Ly-6C	HK1.4	FC
APC anti-mouse Ly-6C	HK1.4	FC
Purified anti-mouse Ly-6C	HK1.4	FC,IHC-F
Biotin anti-mouse Ly-6C	HK1.4	FC
FITC anti-mouse Ly-6C	HK1.4	FC
Alexa Fluor® 647 anti-mouse Ly-6C	HK1.4	FC
PE anti-mouse Ly-6C	HK1.4	FC
PerCP/Cyanine5.5 anti-mouse Ly-6C	HK1.4	FC
PE/Cyanine7 anti-mouse Ly-6C	HK1.4	FC
Alexa Fluor® 488 anti-mouse Ly-6C	HK1.4	FC
Alexa Fluor® 700 anti-mouse Ly-6C	HK1.4	FC
APC/Cyanine7 anti-mouse Ly-6C	HK1.4	FC
PerCP anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 570™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 421™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 510™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 605™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 711™ anti-mouse Ly-6C	HK1.4	FC
Purified anti-mouse Ly-6C (Maxpar® Ready)	HK1.4	FC,CyTOF®
Brilliant Violet 785™ anti-mouse Ly-6C	HK1.4	FC
PE/Dazzle™ 594 anti-mouse Ly-6C	HK1.4	FC
APC/Fire™ 750 anti-mouse Ly-6C	HK1.4	FC
TotalSeq™-A0013 anti-mouse Ly-6C	HK1.4	PG
Brilliant Violet 650™ anti-mouse Ly-6C	HK1.4	FC
TotalSeq™-C0013 anti-mouse Ly-6C	HK1.4	PG
TotalSeq™-B0013 anti-mouse Ly-6C	HK1.4	PG
APC/Fire™ 810 anti-mouse Ly-6C Antibody	HK1.4	FC
Spark UV™ 387 anti-mouse Ly-6C	HK1.4	FC
PE/Fire™ 810 anti-mouse Ly-6C	HK1.4	FC
Spark Blue™ 550 anti-mouse Ly-6C (Flexi-Fluor™)	HK1.4	FC
Spark Blue™ 574 anti-mouse Ly-6C (Flexi-Fluor™)	HK1.4	FC
PE/Cyanine5 anti-mouse Ly-6C	HK1.4	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 07.21.2022

For Research Use Only. Not for diagnostic or therapeutic use.

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