Purified anti-mouse Notch 3 Antibody anti-Notch 3

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Clone: HMN3-133 (See other available formats)
Regulatory Status: RUO
Other Names: Neurogenic locus notch homolog protein 3, Notch-3, CADASIL
Isotype: Armenian Hamster IgG
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130502

500 µg

393 CHF

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Description

The Notch receptors are highly conserved from invertebrates to mammals.While Notch1 and Notch 2 exhibit the highest structural similarity among the four mammalian Notch receptors. Notch 3 has a number of structural and functional differences.The binding of Notch 3 to its ligands results in the proteolysis of Notch and movement of intracellular portions of Notch into the nucleus.This translocation triggers a series of signaling process.Notch 3 is primarily expressed in adult arterial smooth muscle cells.Notch 3 gene mutation can cause CADASIL, an inherited early stroke syndrome.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Notch 3-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_1227735 (BioLegend Cat. No. 130502)

Antigen Details

Structure: Transmembrane receptor, heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
Distribution: Notch 3 is primarily expressed in vascular smooth muscle cells, also found in proliferating neuroepithelium.
Function: Critical role in the structural integrity of distal resistance artieries. Mutations in the Notch 3 receptor on vascular smooth muscles cells is associated with an inherited neurodegenerative disease CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopthy)
Ligand/Receptor: Jag1,2; Delta 1,2
Cell Type: Epithelial cells
Biology Area: Immunology
Antigen References: 1. Ehebauer ME, et al. 2006. Biochem J 392:13
2. Shimizu K, et al. 2000. Mol Cell Biology 20:18
3. Tanigaki K, et al. 2007. Nature Immunol 8:451
4. Bellavia D, et al. 2008. 27:5092
5. Louvi A, et al. Dev Neurosci. 2006. 28:5
Gene ID: 18131 View all products for this Gene ID
UniProt: View information about Notch 3 on UniProt.org

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Description	Clone	Applications
Purified anti-mouse Notch 3	HMN3-133	FC,IHC-F
PE anti-mouse Notch 3	HMN3-133	FC
Alexa Fluor® 647 anti-mouse Notch 3	HMN3-133	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

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