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Purified anti-mouse Plexin B2 Antibody

Pricing & Availability
Clone
3E7 (See other available formats)
Regulatory Status
RUO
Isotype
Armenian Hamster IgG
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Product Citations
publications
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145901 50 µg 124 CHF
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Description

Plexin B2 is a 240 kD transmembrane receptor belonging to the semaphorin receptor family, B subfamily. All the family members share an extracellular semaphorin domain and an intracellular plexin domain-containing tail that can mediate intracellular signaling. Distinct from the other subfamily members, Plexin B2 contains an intracellular domain with a PDZ motif (post synaptic density protein (PSD95), Drosophila disc large tumor suppressor (DlgA), and zonula occludens-1 protein (zo-1)), which can relay extracellular signals to intracellular motifs. Similar to the other B family members, Plexin B2 was also originally found in the nervous system and was later reported on endocrine, reproductive, urinary, digestive, respiratory, and immune systems. In immune cells, Plexin B2 is highly expressed on macrophages, conventional dendritic cells (cDCs), and plasmacytoid dendritic cells (pDCs).  It is also expressed on T cells and T-dependent germinal center B cells. Plexin B2 facilitates ligand induced cell guidance and migration in the nervous system, and induces cytoskeletal changes in overexpression assays through RhoGTPase. However, the function of Plexin B2 in the immune system is not conclusive. Plexin B2 has been reported to modulate upon activation of DCs by TLR ligands, TNF-α, and anti-CD40. It also functions as a negative regulator of Rac and Cdc42 and modulates cell motility in macrophages. Plexin B2 has been found to have several semaphorin ligands including Semaphorin-3E, Semaphorin-4A, Semaphorin-4C, and Semaphorin-4D.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
PAM 2-12 keratinocyets cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Cruz JW, et al. 2019. Sci Rep. 9:12031. PubMed
RRID
AB_2562091 (BioLegend Cat. No. 145901)

Antigen Details

Structure
Contains an extracellular semaphorin domain and an intracellular plexin domain-containing tail; intracellular domain has a PDZ motif (PSD95, DlgA, and zo-1)
Distribution

Macrophages, dendritic cells, T cells, T-dependent germinal center B cells

Function
Involved in cell motility, modulate dendritic cells
Ligand/Receptor
Semaphorin-3E, Semaphorin-4A, Semaphorin-4C, and Semaphorin-4D
Cell Type
B cells, Dendritic cells, Macrophages, T cells
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, Semaphorin Receptors
Antigen References

1. Roney K, et al. 2011. PLoS One 6:e24795.
2. Worzfeld T, et al. 2004. Eur. J. Neurosci. 19:2622.
3. Raper JA, et al. 2000. Curr. Opin. Neurobiol. 10:88.
4. Artigiani S, et al. 2003. J. Bio. Chem. 278:10094.

Gene ID
140570 View all products for this Gene ID
UniProt
View information about Plexin B2 on UniProt.org
Go To Top Version: 1    Revision Date: 05.15.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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