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Purified anti-mouse Podoplanin Antibody

Pricing & Availability
Clone
PMab-1 (See other available formats)
Regulatory Status
RUO
Other Names
T1a, gp38, stromal cell marker
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
PMab-1_Pure_podoplanin_Antibody_092418
Mouse cell line TE-71 was stained with podoplanin (clone PMab-1) purified (filled histogram) or Rat IgG2a, κ isotype control (clone RTK2758) purified (open histogram) followed by Goat anti-rat IgG PE.
  • PMab-1_Pure_podoplanin_Antibody_092418
    Mouse cell line TE-71 was stained with podoplanin (clone PMab-1) purified (filled histogram) or Rat IgG2a, κ isotype control (clone RTK2758) purified (open histogram) followed by Goat anti-rat IgG PE.
  • PMab-1_PURE_Podoplanin_IHC-F_Antibody_091319
    C57 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10µg/ml of purified anti-mouse podoplanin (clone PMab-1) at 4°C overnight. On the next day, tissue was incubate with Alexa Fluor® 594 goat anti-rat IgG (clone Poly4054) antibody (red) for an hour at room temperature. Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X object and stitched with MetaMorph® software.
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156202 500 µg 282 CHF
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Description

The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Peptide (38-51 amino acids of mouse podoplanin)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Yamada S, et al. 2017. Monoclon Antib Immunodiagn Immunother. 36(2):77-79.
Product Citations
  1. Lyras EM, et al. 2022. Elife. 11: . PubMed
  2. Figueiredo CA, et al. 2022. J Neuroinflammation. 19:17. PubMed
RRID
AB_2783132 (BioLegend Cat. No. 156202)

Antigen Details

Structure
43 kD glycosylated type-1 transmembrane protein. Mucin-type protein.
Distribution

Expressed on epithelial and mesothelial cells.

Function
Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation.
Interaction
Interacts with CLEC1B; the interaction is independent of CLEC1B glycosylation and activates CLEC1B; the interaction is dependent of sialic acid on O-glycans.
Ligand/Receptor
CLEC1B
Cell Type
Epithelial cells
Biology Area
Cancer Biomarkers, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Farr A, et al. 1992. J Histochem Cytochem. 40:651.
  2. Schacht V, et al. 2005. Am J Pathol. 166:913.
Gene ID
14726 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org
Go To Top Version: 2    Revision Date: 09.13.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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