Purified anti-mouse/rat CD42d Antibody anti-CD42d

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Clone: 1C2 (See other available formats)
Regulatory Status: RUO
Other Names: Platelet glycoprotein V, GPV, platelet gpV, CD42d antigen, CD42d
Isotype: Armenian Hamster IgG
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Product Citations: publications

C57BL/6 platelets were stained with purified 1C2 (clone 1C2, filled histogram) or Armenian hamster IgG isotype control (bottom), followed by biotinylated anti-Armenian hamster IgG biotin and Sav - PE.

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148501

50 µg

124 CHF

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Description

CD42d is an 83 kD surface glycoprotein that non-covalently associates with GPIb and GPIX to form a receptor complex for von Willebrand factor on megakaryocytes and resting platelets. Binding sites for von Willebrand factor and thrombin have been localized to the GPIba chain of the GPI-b-V-IX complex. Platelet activation with thrombin cleaves the GPI-b-V-IX complex to release a 69 kD soluble fragment. Presence of the GPI-b-V-IX complex is important in Bernard-Soulier syndrome, a rare bleeding disorder.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Mouse platelets
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for relevant formats) include: immunoprecipitation^1,3.
RRID: AB_2564114 (BioLegend Cat. No. 148501)

Antigen Details

Structure: 83 kD heterodimer composed of 2 polypeptide chains, an α chain (GP1BA) and a β chain (GP1BB).
Distribution: Resting platelets and megakaryocytes.
Function: CD42d is a component of the GPIb-V-IX complex, functions as a vWF receptor, and mediates vWF-dependent platelet adhesion to blood vessels.
Ligand/Receptor: CD42d.
Cell Type: Megakaryocytes, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Sivaraman B, et al. 2011. Biomaterials. 32:5365.
2. Berger G, et al. 1996. Blood. 87:1385.
3. Ravanat C, et al. 1997. Blood. 89:3253.
4. Andrews RK, et al. 1998. Biochemistry. 37:638.
5. Inoue O, et al. 2008. 283:16279.
6. Hickey MJ, et al. 1993. Proc. Natl. Acad. Sci. 90:8327.
7. Shimomura T, et al. 1990. Blood. 75:2349.
8. Wu G, et al. 1997. Blood. 90:2660.
Gene ID: 14729 View all products for this Gene ID
UniProt: View information about CD42d on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications
Purified anti-mouse/rat CD42d	1C2	FC,IP
PE anti-mouse/rat CD42d	1C2	FC
APC anti-mouse/rat CD42d	1C2	FC
PerCP/Cyanine5.5 anti-mouse/rat CD42d	1C2	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 08.12.2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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