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Purified anti-mouse TLR4 (CD284)/MD2 Complex Antibody

Pricing & Availability
Clone
MTS510 (See other available formats)
Regulatory Status
RUO
Other Names
Toll-like Receptor 4/MD2 complex, TLR4-MD2-complex, TLR4/MD2, CD284/MD2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a-MTS510_Pure_091907
Mouse TLR4/MD2 transfected cells were stained with purified CD284 (clone MTS510) (filled histogram), or purified rat IgG2a, κ (open histogram), followed by anti-rat IgG FITC.
  • a-MTS510_Pure_091907
    Mouse TLR4/MD2 transfected cells were stained with purified CD284 (clone MTS510) (filled histogram), or purified rat IgG2a, κ (open histogram), followed by anti-rat IgG FITC.
  • b-TLR4_MD2_Mouse-Gut_071212
    FFPE mouse gut was stained with Purified Anti-Mouse TLR4/MD2 Complex (Cat# 117602) or Purified Rat IgG2a, κ at 1:50 (10 µg/mL) using 10 mM Sodium Citrate Buffer pH 6.0 for antigen retrieval and ABC detection method. Cellular localization: Cell Membrane.
    Top: Positive Ctrl: FFPE Mouse Gut, 20X Magnification; Bottom: Negative Ctrl: FFPE Mouse Gut, 20X Magnification
  • c-TLR4_MD2_Mouse-Gut-Neg-Ctrl_071212
    FFPE mouse gut was stained with Purified Anti-Mouse TLR4/MD2 Complex (Cat# 117602) or Purified Rat IgG2a, κ at 1:50 (10 µg/mL) using 10 mM Sodium Citrate Buffer pH 6.0 for antigen retrieval and ABC detection method. Cellular localization: Cell Membrane.
    Top: Positive Ctrl: FFPE Mouse Gut, 20X Magnification; Bottom: Negative Ctrl: FFPE Mouse Gut, 20X Magnification
  • d-MTS510_PURE_TLR4_Antibody_4_112520
    Western blot analysis of Raw264.7 untreated (lane 1), Raw264.7 starved overnight and treated with 1 µg/mL lipopolysaccharide (LPS) for one day (lane 2), peripheral blood mononuclear cells (PBMCs) untreated (lane 3), and PBMCs activated with CD3E (clone OKT3) (10 µg/mL coated on plate)/CD28 (clone CD28.2) (2 µg/mL) antibody for two days using anti-mouse TLR4 (CD284)/MD2 Complex (clone MTS510) antibody followed by HRP goat anti-rat IgG antibody. Purified anti-β-actin (clone Poly6221) antibody was used as loading control.
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117601 50 µg 112 CHF
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117602 200 µg 235 CHF
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Description

Toll-like receptors are highly conserved from Drosophila to humans and share structural and functional similarities. They are type I transmembrane signaling receptors which activate the innate immune system in response to pathogen invasion. So far, at least 13 TLR members have been identified. The secretory protein, MD2, associates with cell-surface bound TLR4 (CD284) on monocytes, B cells and T cells. TLR4 has been recognized as critical for host recognition of bacterial LPS in conjunction with MD2.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ba/F3 cell line transfected with mouse TLR4 /MD2 complex
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P, WB - Verified
ICFC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.8 Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.8 In addition to flow cytometric uses, the MTS510 antibody has also been used for immunoprecipitation, immunofluorescence, and blocking of LPS-induced stimulation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 117607). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 117612) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Akashi S, et al. 2000. J. Immunol. 164:3471. (FC, ICFC, IP, Block)
  2. Kawasaki K, et al. 2003. J. Immunol. 170:413. (FC)
  3. Nalbandian G, et al. 2005. J. Immunol. 175:2666. (FC) PubMed
  4. Kanada S, et al. 2011. Blood 117:2211. PubMed
  5. Laplate P, et al. 2011. J. Biol Chem. 286:42494. PubMed
  6. Khan MA, et al. 2006. Infect. Immun. 74:2522. (IF)
  7. Tanigawa T, et al. 2013. Innate Immun. 19:115. PubMed
  8. Akashi S, et al. 2003. J. Exp. Med. 198:1035.
Product Citations
  1. Ricci-Azevedo R, et al. 2021. Front Immunol. 12:655371. PubMed
  2. Takimoto Y, et al. 2023. iScience. 26:106220. PubMed
  3. Suzuki M, et al. 2009. J Immunol. 182:6485. PubMed
  4. Laplante P, et al. 2011. J Biol Chem. 286:42494. PubMed
  5. Shimizu J, et al. 2022. Front Immunol. 13:903859. PubMed
  6. Yang H, et al. 2009. PLoS One. 4:e6520. PubMed
  7. Long C, et al. 2012. J Leukoc Biol. 92:667. PubMed
  8. Iwata Y, et al. 2009. Am J Pathol. 175:649. PubMed
  9. Mao A, et al. 2005. J Immunol. 175:5146. PubMed
  10. Saxena A, et al. 2011. Cardiovasc Res. 91:350. PubMed
  11. Sheng K, et al. 2010. J Immunol. 184:2863. PubMed
  12. Suzuki H, et al. 2020. J Immunol. 205:811. PubMed
  13. Bhattacharyya S, et al. 2007. Am J Physiol Gastrointest Liver Physiol. 293:G429. PubMed
  14. Sheng K, et al. 2008. J Immunol. 181:2455. PubMed
RRID
AB_313788 (BioLegend Cat. No. 117601)
AB_313788 (BioLegend Cat. No. 117602)

Antigen Details

Structure
Type I transmembrane protein complex
Distribution

Monocytes, B cells and T cells

Function
Activates the innate immune system against Gram-negative bacterial pathogens by recognition of LPS
Ligand/Receptor
Bacterial LPS
Cell Type
B cells, Dendritic cells, Monocytes, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Frleta D, et al. 2003. J. Leukocyte Biol. 74:1064.

Gene ID
21898 View all products for this Gene ID
UniProt
View information about TLR4 MD2 Complex on UniProt.org

Related FAQs

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Go To Top Version: 6    Revision Date: 11.25.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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