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Purified anti-mouse Trem-like 4 (Treml4) Antibody

Pricing & Availability
Clone
16E5 (See other available formats)
Regulatory Status
RUO
Other Names
Trem-l4, TREM like transcript 4, triggering receptor expressed on myeloid cells-like 4
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
16E5_PE_010412
C57BL/6 mouse splenocytes were stained with Treml4 (clone 16E5) PE (filled histogram) or rat IgG1, κ PE isotype control (open histogram). Data shown was from gated on CD8+CD11c+ cells.
  • 16E5_PE_010412
    C57BL/6 mouse splenocytes were stained with Treml4 (clone 16E5) PE (filled histogram) or rat IgG1, κ PE isotype control (open histogram). Data shown was from gated on CD8+CD11c+ cells.
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143302 100 µg 229 CHF
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Description

Trem-like 4, a type I transmembrane protein and a member of the TREM family, consists of a single extracellular Ig-like domain of the V-type, a transmembrane domain, and a short cytoplasmic domain. Trem-like 4 is expressed on macrophages in the spleen, and dendritic cells, especially CD8+ dendritic cells. It is proposed to regulate the immune response based on its expression profile. It has been reported that B7-H3 is a receptor and that the soluble form of Treml4 has a binding affinity to late apoptotic or necrotic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Extracellular domain of recombinant mouse Trem-like 4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescent staining, immunoprecipitation, and Western blotting1.

Application References

(PubMed link indicates BioLegend citation)
  1. Hemmi H, et al. 2009. J. Immunol. 182:1278. (WB)
Product Citations
  1. Lilly CL, et al. 2017. Mol Ther Oncolytics. 31:4. PubMed
RRID
AB_11149169 (BioLegend Cat. No. 143302)

Antigen Details

Structure
Type I transmembrane protein; member of the TREM family
Distribution

CD8+ dendritic cells, dendritic cell subsets, macrophages in the spleen, myeloid cells

Function
Regulates innate immune response and homeostatis
Interaction
Associates with DAP12; binds late apoptotic cells/necrotic cells
Cell Type
B cells, Dendritic cells, Macrophages
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Ford JW and McVicar DW. 2009. Curr. Opin. Immunol. 21:38.
2. Hemmi H, et al. 2009. J. Immunol. 182:1278.

Gene ID
224840 View all products for this Gene ID
UniProt
View information about Trem-like 4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04.08.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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