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Purified anti-mouse VISTA (PD-1H) Antibody

Pricing & Availability
Clone
MIH63 (See other available formats)
Regulatory Status
RUO
Other Names
PD-1 homolog, Dies1, B7-H5
Isotype
Rat IgG2a, κ
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Product Citations
publications
MIH63_PURE_VISTA_Antibody_FC_1_060315
C57BL/6 mouse splenocytes were stained with purified PD-1H (clone MIH63, top) or purified rat IgG2a, κ isotype control (bottom) followed by anti-rat IgG PE. The cells were then stained with CD8 APC.
  • MIH63_PURE_VISTA_Antibody_FC_1_060315
    C57BL/6 mouse splenocytes were stained with purified PD-1H (clone MIH63, top) or purified rat IgG2a, κ isotype control (bottom) followed by anti-rat IgG PE. The cells were then stained with CD8 APC.
  • MIH63_PURE_VISTA_Antibody_FC_2_060315
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150202 100 µg 118 CHF
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Description

PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein that is composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and natural killer cells (NK). It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants due to its ability to preferentially suppress CD4+ T cell-mediated immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse VISTA transfected J558 cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Muhammad F, et al. 2020. J Autoimmun. 111:102441. PubMed
  3. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
RRID
AB_2565897 (BioLegend Cat. No. 150202)

Antigen Details

Structure
Type I transmembrane protein, member of the CD28 family.
Distribution

Macrophages, dendritic cells, neutrophils, natural killer cells, and naive and activated T cells.

Function
Negative regulator of the immune response.
Cell Type
Dendritic cells, Macrophages, Neutrophils, NK cells, T cells
Biology Area
Immunology, Inhibitory Molecules
Antigen References

1. Flies DB, et al. 2011. J. Immunol. 187:1537.
2. Wang Li, et al. 2011. J. Exp. Med. 208:577.
3. Flies DB, et al. 2014. J. Clin. Invest. 124:1966.

Gene ID
74048 View all products for this Gene ID
UniProt
View information about VISTA on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06.22.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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