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Purified anti-MRP-14 (S100A9) Antibody

Pricing & Availability
Clone
A15105J (See other available formats)
Regulatory Status
RUO
Other Names
S100A9, Migration inhibitory factor-related proteins-14, Calgranulin-B, Leukocyte L1 complex heavy chain, Calprotectin L1H subunit
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A15105J_PURE_MRP-14_Antibody_WB_010218
Recombinant human S100A8 protein (Cat. No. 719902, lane 1), S100A9 protein( lane 2) and total lysates (15 µg protein) from HeLa (negative control, lane 3) , PBMC (lane 4) were resolved by 4-20% Tris-Glycine electrophoresis, transferred to nitrocellulose, and probed with 1:5000 (0.1 µg/mL) diluted purified anti-MRP-14 (S100A9) (clone A10105J). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • A15105J_PURE_MRP-14_Antibody_WB_010218
    Recombinant human S100A8 protein (Cat. No. 719902, lane 1), S100A9 protein( lane 2) and total lysates (15 µg protein) from HeLa (negative control, lane 3) , PBMC (lane 4) were resolved by 4-20% Tris-Glycine electrophoresis, transferred to nitrocellulose, and probed with 1:5000 (0.1 µg/mL) diluted purified anti-MRP-14 (S100A9) (clone A10105J). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control (lower). Lane M: Molecular weight ladder.
Cat # Size Price Quantity Check Availability Save
600301 25 µg 101 CHF
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600302 100 µg 253 CHF
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Description

MRP-14, also known as S100A9, belongs to the S100 family of proteins containing 2-EF-hand calcium-binding motifs. It forms non-covalently associated complexes with another S100 family member, MRP-8. MRP14 is expressed in neutrophils and monocytes; it also has been found in ileal tissue eosinophils. The S100A8/A9 complex has been shown to play a role during calcium-dependent activation as well as have antibacterial, antifungal, immunomodulating, and anti-proliferative effects. It also functions as a chemoattractant factor for neutrophils and has been considered an endogenous activator of Toll-like receptor 4. S100A9 has been identified as a marker for CD14(+) HLA-DR(-/low) MDSCs.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human S100A8/S100A9 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.5 µg per mL (1:1000-1:10000). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not recognize S100A8. The 114 amino acid recombinant S100A9 protein has a predicted Mw of ~13 kD for monomer and ~26 kD for dimer.

Product Citations
  1. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
RRID
AB_2721746 (BioLegend Cat. No. 600301)
AB_2721746 (BioLegend Cat. No. 600302)

Antigen Details

Structure
Belongs to S100 family of proteins containing 2-EF-hand calcium -binding motifs, forms non-covalently associated complexes with another S100 family member MRP-8. S100A9 has 114 amino acids with the predicted Mw of ~13 kDa.
Distribution

MDSCs, neutrophils, monocyte, and ileal tissue eosinophils

Function
Calcium-dependent activation, immunomodulating and antiproliferative, endogenous activator of Toll-like receptor 4
Interaction
Calcium, S100A8
Ligand/Receptor
RAGE, TLR4
Cell Type
Neutrophils, Monocytes, Eosinophils
Biology Area
Cancer Biomarkers, Cell Biology, Immunology, Innate Immunity
Antigen References

1. Loser K, et al. 2010. Nat. Med. 16:713.
2. Cheng P, et al. 2008. J. Exp. Med. 205:2235.
3. Leukert N, et al. 2006. J. Mol. Biol. 359:961-72.
4. Lagasse E, et al. 1988. Mol. Cell. Biol. 8:2402.
5. Zhao F, et al. 2012. Immunology. 136:176.

Gene ID
6280 View all products for this Gene ID
UniProt
View information about MRP-14 on UniProt.org
Go To Top Version: 1    Revision Date: 01.03.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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