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Purified anti-MYDGF (SF20) Antibody

Pricing & Availability
Clone
W16077A (See other available formats)
Regulatory Status
RUO
Other Names
Myeloid-derived growth factor, Interleukin-25 (IL-25), Stromal cell-derived growth factor SF20,
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1_W16077A_PURE_MYDGF_Antibody_WB_1_031317
Total cell lysates (15 µg protein) from Molt-4, HeLa and NIH3T3 were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 2 µg/mL purified anti-MYDGF (SF20) antibody (clone W16077A) antibody (upper). Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M: MW ladder.
  • 1_W16077A_PURE_MYDGF_Antibody_WB_1_031317
    Total cell lysates (15 µg protein) from Molt-4, HeLa and NIH3T3 were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 2 µg/mL purified anti-MYDGF (SF20) antibody (clone W16077A) antibody (upper). Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M: MW ladder.
  • 2_W16077A_PURE_MYDGF_Antibody_WB_2_111617
    Total lysates (15 µg protein) from 293T and 293T/MYDGF CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:250 diluted (2 µg/mL) purified anti-MYDGF (SF20) antibody, clone W16077A (upper) or 1:3000 diluted anti-GAPDH antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-rat-IgG secondary antibody conjugated to HRP for anti-MYDGF (SF20) antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M: Molecular weight ladder. Non-specific bands were observed over 150 kD in the 293T WT and KO cell lines tested.
  • 3_W16077A_PURE_MYDGF_Antibody_IF_1_111617_Updated
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 0 and 2 µg/ml anti-MYDGF (SF20) antibody (clone W16077A) overnight at 4° followed by Alexa Fluor® 594 (red) conjugated goat anti-rat IgG for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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695802 100 µg 276 CHF
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Description

Myeloid-derived growth factor (MYDGF), also known as Stromal cell-derived growth factor SF20, is encoded by an open reading frame on chromosome 19 (C19orf10) and is secreted by bone marrow-derived monocytes and macrophages. MYDGF protects cardiomyocytes from apoptosis in a PI3K/AKT-dependent signaling pathway and promotes angiogenesis in endothelial cells through a MAPK1/3, STAT3, and CCND1-mediated signaling. During myocardial infarction, MYDGF therapy has been found to increase angiogenesis in the infarcted area and attenuate infarct size, scar formation, cardiac dilatation, and systolic dysfunction. In comparison to wild-type mice, MYDGF-deficient mice developed larger infarcts and had more severe systolic dysfunction.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human MYDGF recombinant protein (32-173 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, KO/KD-WB - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W16077A does not cross-react with mouse (in-house tested). As per the Human Protein Atlas RNA level dataset, Molt-4 is expected to have a low level of MYDGF expression.

RRID
AB_2650929 (BioLegend Cat. No. 695802)

Antigen Details

Structure
142 amino acids with a predicted molecular weight of 15.8 kD.
Distribution

Secreted

Function
MYDGF is a secreted protein and functions in promoting survival of cardiomyocytes and angiogenesis in endothelial cells.
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology
Antigen References

1. Dwivedi RC, et al. 2016. Proteomics Clin. Appl. 10:663.
2. Cully M. 2015. Nat. Rev. Drug Discov. 14:164.
3. Korf-Klingebiel M, et al. 2015. Nat. Med. 21:140.

Gene ID
56005 View all products for this Gene ID
UniProt
View information about MYDGF on UniProt.org
Go To Top Version: 0    Revision Date: 03.15.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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