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Purified anti-Myelin CNPase Antibody

Pricing & Availability
Clone
SMI 91 (See other available formats)
Regulatory Status
RUO
Other Names
CNPase, Cnp-1, Cnp1, 2',3'-cyclic-nucleotide 3'-phosphodiesterase
Isotype
Mouse IgG1, κ
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Product Citations
publications
1-SMI91_PURE_Myelin_CNPase_MB_Antibody_042518
IHC staining of purified anti-Myelin CNPase antibody (clone SMI 91) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 10X (Cat. No. 927801), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1-SMI91_PURE_Myelin_CNPase_MB_Antibody_042518
    IHC staining of purified anti-Myelin CNPase antibody (clone SMI 91) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 10X (Cat. No. 927801), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 2-SMI91_PURE_Myelin_CNPase_RB_Antibody_042518
    IHC staining of purified anti-Myelin CNPase antibody (clone SMI 91) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 10X (Cat. No. 927801), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3-SMI91_PURE_Myelin_CNPase_Antibody_042518
    Western blot of purified anti-Myelin CNPase antibody (clone SMI 91). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 30 µg of mouse brain lysate; Lane 4: 30 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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836403 25 µg 112 CHF
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836404 100 µg 276 CHF
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Description

High CNPase expression is seen in myelin producing cells, including oligodendrocytes and Schwann cells. CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (CNS). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth. The enzyme isolated from the mammalian brain is primarily a mixed dimer of approximately 94 kD. The dimer consists of a varied proportion of CNP1 (46 kD) and CNP2 (48 kD) subunits in various species. Since the enzyme is a myelin-associated enzyme, it is of considerable interest in the study of diseases and disorders in which myelin is affected, such as multiple sclerosis, subacute sclerosing panencephalitis, acquired immunodeficiency with CNS involvement, and peripheral neuropathies. The combination of clone SMI 91 with clone SMI 94 and/or clone SMI 99 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
IHC-F, ICC, EM, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 1.0 µg/mL. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissue sections1,2,4,8 (IHC), immunocytochemistry5,6 (ICC), and western blot (WB).3,7

Application References

(PubMed link indicates BioLegend citation)
  1. Samanta J, et al. 2010. Stroke. 2:357-62. (IHC-F) 
  2. Werner HB, et al. 2007. J. Neurosci. 27:7717-7730. (IHC-P) PubMed
  3. McFerran B, et al. 1997. J Cell Sci. 110:2979-2985. (WB)
  4. Trolle C, et al. 2014. BMC Neurosci. 15:60. (IHC-F) PubMed
  5. Liyanage VR, et al. 2013. Mol. Autism 4:46. (ICC)
  6. Göttle P, et al. 2015. J. Neurosci. 35:906. (ICC)
  7. Yu C, et al. 2008. Glia. 56:877. (WB)
  8. Johnson GC, et al. 2014. Pathol. 51:146. (IHC-P) PubMed
Product Citations
  1. Huang S, et al. 2021. STAR Protocols. 2(2):100416. PubMed
  2. Kato Y, et al. 2022. Noncoding RNA. 8: . PubMed
  3. Ritzel RM, et al. 2023. Sci Adv. 9:eadd1101. PubMed
  4. Dustin E, et al. 2023. Biomedicines. 11:. PubMed
  5. Zhao X, et al. 2021. iScience. 24:103485. PubMed
  6. Li AM, et al. 2021. Cereb Cortex. 31:4340. PubMed
  7. Chew LJ, et al. 2020. Cell Reports. 29(10):3173-3186.e7.. PubMed
  8. Shi Y, et al. 2019. J Exp Med. 216:2546. PubMed
  9. Martín–López E, et al. 2018. Brain Struct Funct. 223:4067. PubMed
  10. Stojic A et al. 2018. Glia. 67(3):512-524 . PubMed
  11. Werner H, et al. 2007. J Neurosci. 27:7717-7730. PubMed
  12. Huerga-Gómez A, et al. 2020. Glia. . PubMed
  13. Rahman A et al. 2018. The Journal of comparative neurology. 527(4):797-817 . PubMed
  14. Schwabenland M, et al. 2021. Immunity. . PubMed
  15. Hill RA, et al. 2018. Nat Neurosci. 21:683. PubMed
  16. Jablonska B, et al. 2022. Nat Commun. 13:4771. PubMed
  17. Samanta J, et al. 2010. Stroke. 41:357-362. PubMed
RRID
AB_2566639 (BioLegend Cat. No. 836403)
AB_2566639 (BioLegend Cat. No. 836404)

Antigen Details

Structure
Reacts with the 46 kD and 48 kD subunits of the 94 kD myelin CNPase dimer.
Distribution

Tissue distribution: Central and peripheral nervous systems.
Cellular distribution: Cytoskeleton, nucleus, mitochondria, cytosol, and plasma membrane.

Function
CNPase detects developing and adult myelin, and developing oligodendrocytes and Schwann cells. CNPase distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections.
Interaction
Detects developing and adult myelin and developing oligodendrocytes and Schwann cells. Distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections.
Cell Type
Oligodendrocytes
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Enzymes and Regulators, Phospho-Proteins
Antigen References
  1. Han H, et al. 2013. Biofactors. 39(3):233.
  2. Raasakka A, et al. 2014. Neurosci Bull. 30(6):956.
  3. Kim JY, et al. 2003. J. Neurosci. 23(13):5561-5571.
  4. Sprinkle TJ, et al. 1989. Crit. Rev. Neurobiol. 4:235-301.
  5. Ding BS, et al. 2013. PLoS One 8:e62150.
  6. Ferletta M, et al. 2011. Int. J. Cancer 129:45.
Gene ID
1267 View all products for this Gene ID
UniProt
View information about Myelin CNPase on UniProt.org
Go To Top Version: 2    Revision Date: 09.25.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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