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Purified anti-NAC-1 Antibody

Pricing & Availability
Clone
SWN-3 (See other available formats)
Regulatory Status
RUO
Other Names
Nucleus accumbens-associated protein 1, NACC1, BTB/POZ domain-containing protein 14B
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SWN-3_PURE_NAC-1_Antibody_1_IHC-P_041519
IHC staining of purified anti-NAC-1 antibody (clone SWN-3) on formalin-fixed, paraffin-embedded human brain cortex tissue. Following antigen retrieval using sodium citrate, the tissue was incubated with 10 µg/mL of the primary antibody for 24 hours at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit was used for detection, follow by counterstaining with hematoxylin.
  • SWN-3_PURE_NAC-1_Antibody_1_IHC-P_041519
    IHC staining of purified anti-NAC-1 antibody (clone SWN-3) on formalin-fixed, paraffin-embedded human brain cortex tissue. Following antigen retrieval using sodium citrate, the tissue was incubated with 10 µg/mL of the primary antibody for 24 hours at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit was used for detection, follow by counterstaining with hematoxylin.
  • SWN-3_PURE_NAC-1_Antibody_2_WB_013117
    Western blot of purified anti-NAC-1 antibody (clone SWN-3). Lane 1: molecular weight marker, Lane 2: 20 µg of SH-SY5Y cell lysate. The blot was incubated with the primary antibody at 5 µg/ml overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.
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849301 25 µg 106 CHF
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849302 100 µg 265 CHF
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Description

NAC-1 is a member of the POZ/BTB protein family involved in several cellular processes including proliferation, apoptosis and transcription regulation. NAC-1 is required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant NAC-1 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
FC, ICC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: flow cytometric analysis1, immunofluorescence microscopy1, and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakayama K, et al. 2006. Proc. Natl. Acad. Sci. USA 103(49):18739. (IP, WB, IHC, IF) PubMed
Product Citations
  1. Wang L, et al. 2022. Viruses. 14: . PubMed
RRID
AB_2650705 (BioLegend Cat. No. 849301)
AB_2650705 (BioLegend Cat. No. 849302)

Antigen Details

Structure
NAC-1 is a 527 amino acid protein with an apparent molecular mass of 57.3 kD.
Distribution

Tissue distribution: brain, ovary, testis, and thyroid.
Cellular distribution: nucleus and cytosol.

Function
NAC-1 is a transcriptional repressor that plays a role in stem cell self-renewal and pluripotency maintenance. NAC-1 also contributes to the progression of multiple types of cancer by suppressing the transcription of the tumor suppressor Gadd45GIP1. It also functions as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4.
Ligand/Receptor
Interacts with other POZ/BTB proteins, HDACs, CUL3, PSMD7, RCOR1.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Antigen References

1. Mackler SA, et al. 2000. J. Neurosci. 20:6210-7. PuMed
2. Korutla L, et al. 2009. Neurochem. Int. 54:245-52. PubMed
3. Korutla L, et al. 2003. Neurosci. Res. 46(1):33-9. PubMed

Gene ID
112939 View all products for this Gene ID
UniProt
View information about NAC-1 on UniProt.org

Other Formats

View All NAC-1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-NAC-1 SWN-3 IHC-P,WB,FC,ICC,IP
Go To Top Version: 2    Revision Date: 05.26.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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