- Clone
- Poly22A17 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Nes
- Isotype
- Rabbit Polyclonal IgG
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- Product Citations
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Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
626351 | 50 µL | 305 CHF | ||||
626352 | 200 µL | 725 CHF |
Nestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Recombinant fragment of human nestin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICC - Quality tested
WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a dilution range of 1:50 - 1:1000 is recommended. For western blotting, the suggested dilution range of this reagent is 1:5000 - 1:50000. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For Immunocytochemistry, either 4% paraformaldehyde/Triton X-100, 4% paraformaldehyde/methanol, or methanol only is recommended for fixation/permeabilization.
- RRID
-
AB_3097319 (BioLegend Cat. No. 626351)
AB_3097319 (BioLegend Cat. No. 626352)
Antigen Details
- Structure
- 1621 amino acids with predicted molecular weight of 177 kD; the observed molecular weight is larger than 250 kD
- Distribution
-
Cytosol/Neural stem cells and various cancer cells
- Function
- Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation
- Interaction
- Vimentin, alpha-internexin and FHOD3
- Cell Type
- Neural Stem Cells
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Antigen References
-
- Matsuda Y, et al. 2013. World J Gastroenterol. 19:42-8.
- Matsuda Y, et al. 2012. Med Mol Morphol. 45:59-65.
- Krupkova O Jr, et al. 2010. Neoplasma. 57:291-8.
- Hombach-Klonisch S, et al. 2008. Arch Immunol Ther Exp (Warsz). 56:165-80.
- Pilkington GJ. 2005. Cell Prolif. 38:423-33.
- Gene ID
- 10763 View all products for this Gene ID
- UniProt
- View information about Nestin on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Nestin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Nestin | Poly22A17 | ICC,WB |
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