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- Clone
- SMI 33 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, neurofilament triplet H protein
- Isotype
- Mouse IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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IHC staining of purified anti-Neurofilament H & M (NF-H/NF-M), Non-Phosphorylated antibody (clone SMI 33) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using BioLegend’s Retrieve-ALL 3 pH 4.8 the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Kit was used for detection, followed by counterstaining with hematoxylin. -
Western blot of purified anti-Neurofilament H & M (NF-H/NF-M), Non-Phosphorylated antibody (clone SMI 33). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.25 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 835403 | 25 µg | 112 CHF | ||||
| 835404 | 100 µg | 276 CHF | ||||
Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and to regulate the axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest NF (NF-L) runs at 68-70 kD. The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from the glia (negative for NF).
Product DetailsProduct Details
- Verified Reactivity
- Rat, Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Immunohistochemistry. For Immunohistochemistry of paraffin-embedded tissues, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Cell bodies and dendrites are generally unstained. Other cells and tissues are unreactive except for peripheral axons.
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Application References
(PubMed link indicates BioLegend citation) -
- Wang J, et al. 2001. FEBS Lett. 19;507(1): 81-7. (WB)
- Product Citations
-
- RRID
-
AB_2716099 (BioLegend Cat. No. 835403)
AB_2716099 (BioLegend Cat. No. 835404)
Antigen Details
- Structure
- The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD.
- Distribution
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Tissue Distribution: CNS, peripheral nerves and glandular cells of the prostate
Cellular distribution: cytoskeleton, nucleus, cytosol, and mitochondrion - Function
- Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter.
- Cell Type
- Mature Neurons
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments, Phospho-Proteins
- Gene ID
- 4744 View all products for this Gene ID
- UniProt
- View information about Neurofilament HM NF-H NF-M on UniProt.org
Other Formats
View All Neurofilament H&M (NF-H/NF-M) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Neurofilament H & M (NF-H/NF-M), Non-Phosphorylated | SMI 33 | IHC-P,WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Neurofilament H & M (NF-H/NF-M), Non-Phosphorylated
IHC staining of purified anti-Neurofilament H & M (NF-H/NF-M... 
Western blot of purified anti-Neurofilament H & M (NF-H/NF-M...
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