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Purified anti-Neurofilament L (NF-L) Antibody

Pricing & Availability
Clone
NFL2 (See other available formats)
Regulatory Status
RUO
Other Names
Neurofilament Light Polypeptide 68kD, Neurofilament Light Polypeptide, Neurofilament Subunit NF-L, CMT1F, NFL, NEFL, NF-L
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
NFL2_Pure_NeurofilamentL_Antibody_0_092619
Western blot of purified anti-Neurofilament L (NF-L) antibody (clone NFL2). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • NFL2_Pure_NeurofilamentL_Antibody_0_092619
    Western blot of purified anti-Neurofilament L (NF-L) antibody (clone NFL2). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • NFL2_Pure_NeurofilamentL_Antibody_1_092619
    Western blot of purified anti-Neurofilament L (NF-L) antibody (clone NFL2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain cerebellum lysate; Lane 3: 20 µg of human brain cortex lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • NFL2_Pure_NeurofilamentL_Antibody_2_IHC_041719
    IHC staining of purified anti-Neurofilament L (NF-L) antibody (clone NFL2) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
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846002 100 µg 265 CHF
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Description

Neurofilaments (NF) are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Detection of NF, and the subunits of NF, may serve as a biomarker for axonal degeneration. The degree of axonal degeneration is related to the amount of NF detected in the cerebrospinal fluid (CSF) and the blood. Neurofilament light (NF-L) levels are elevated in Alzheimer’s Disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified macaque NFL.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10.0 µg/ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 1.0 - 5.0 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Park YG, et al. 2018. Nat Biotechnol. 37:73. PubMed
RRID
AB_2566775 (BioLegend Cat. No. 846002)

Antigen Details

Structure
Neurofilament light is a 68 kD protein.
Distribution

Cytoskeleton and the cytoplasm.

Function
Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites.
Cell Type
Mature Neurons
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Zetterberg H, Blennow K. 2015. JAMA Neurol. 11:1237-8.
2. Bäckström D, et al. 2015. JAMA Neurol. 72(10):1175.
3. Janelidze S, et al. 2015. Neurology 85:1834.
4. Magdalinou NK, et al. 2015. J. Neurol. Neurosurg. Psychiatry 86(11):1240.
5. Skillbäck T, et al. 2014. Neurology. 83:1945.

Gene ID
4747 View all products for this Gene ID
UniProt
View information about Neurofilament L on UniProt.org
Go To Top Version: 3    Revision Date: 09.26.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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