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Purified anti-Neurogranin (NRGN) Antibody

Pricing & Availability
Clone
NG2 (See other available formats)
Regulatory Status
RUO
Other Names
Ng, RC3, Calmodulin-Binding Protein, Protein Kinase C Substrate, Hng
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
NG2_PURE_Neurogranin_NRGN_1_IHC_041719
IHC staining of purified anti-Neurogranin (NRGN) antibody (clone NG2) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • NG2_PURE_Neurogranin_NRGN_1_IHC_041719
    IHC staining of purified anti-Neurogranin (NRGN) antibody (clone NG2) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • NG2_PURE_Neurogranin_NRGN_2_WB_071619
    Western blot of purified anti-Neurogranin (NRGN) antibody (clone NG2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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845602 100 µg 265 CHF
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Description

Neurogranin (NRGN) is the main postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. It is suggested that the NRGN is a direct target for thyroid hormone in the human brain, and that the control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects. Neurogranin in CSF has been implicated as a possible marker for synaptic degeneration in Alzheimer’s disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH conjugated peptide corresponding to residues 52-78 of human neurogranin (NRGN).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The epitope has been mapped to a.a. 52-63 of human NRGN.

Product Citations
  1. Park JS, et al. 2023. Nature. 377:617. PubMed
  2. Wang M, et al. 2021. Neuron. 109(10):1636-1656.e8. PubMed
RRID
AB_2566622 (BioLegend Cat. No. 845602)

Antigen Details

Structure
78 amino acids with a molecular weight of 7.6 kD.
Distribution

In the cerebral cortex, NRGN is found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. It is not found in the dendrites in patients with Alzheimer's disease.

Function
Neurogranin is a neuron-specific protein kinase C substrate (post synaptic protein) whose main function is to bind CaM (calmodulin) in the absence of Ca2+. Neurogranin modulates the homeostasis of Ca2+ in neurons and its role has been implicated in learning and memory.
Ligand/Receptor
Calmodulin-binding.
Cell Type
Neurons
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References

1. Zhong L, et al. 2015. J. Neurosci. 35:7503.
2. Huang K, et al. 2004. J. Neurosci. 24:10660.
3. Dominguez-Gonzalez I, et al. 2007. Biochem. J. 404:31.
4. Prichard L, et al. 1999. J. Biol. Chem. 274:7689.

Gene ID
4900 View all products for this Gene ID
UniProt
View information about Neurogranin on UniProt.org
Go To Top Version: 2    Revision Date: 07.16.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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