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- Clone
- NG7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ng, RC3, Calmodulin-Binding Protein, Protein Kinase C Substrate, Hng
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
IHC staining of Clone NG7 on formalin fixed paraffin embedded rat brain tissue at 5 µg/ml. Sodium citrate antigen retrieval was used and followed by incubation of the primary antibody for 60 minutes at room temperature. The Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) was used for detection. -
Western blot analysis of Clone NG7. Lane 1: Molecular weight marker; Lane 2: Rat brain lysate; Lane 3: Human cortex lysate. Blot was incubated at 1 µg/ml for 24 hours at 4°C, followed by incubation with a fluorescent labeled goat anti-mouse secondary antibody.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 845702 | 100 µg | 265 CHF | ||||
Neurogranin (NRGN) is the main postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. It is suggested that the NRGN is a direct target for thyroid hormone in the human brain, and that the control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects. Neurogranin in CSF has been implicated as a possible marker for synaptic degeneration in Alzheimer’s disease.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH conjugated peptide corresponding to residues 52-78 of human NRGN.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
WB - Verified
IP, ELISA Capture - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Tissue Sections: Formalin-fixed, paraffin-embedded tissue sections
Primary Antibody Incubation: 20 minutes at room temperature
Antigen Retrieval: Sodium citrate pH 6 (Cat# 928501)
Positive Control: Rat or human brain tissue - Application Notes
-
Additional reported applications (for the relevant formats of this clone) include: immunoprecipitation and ELISA capture.
The epitope has been mapped to a.a. 52-63 of human NRGN. -
Application References
(PubMed link indicates BioLegend citation) -
- Kvartsberg H, et al. 2014. Alzheimers. Dement. 10:1180-90.
- RRID
-
AB_2566623 (BioLegend Cat. No. 845702)
Antigen Details
- Structure
- 78 amino acids with a molecular weight of 7.6 kD.
- Distribution
-
In the cerebral cortex, NRGN is found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. It is not found in the dendrites in patients with Alzheimer's disease.
- Function
- Neurogranin is a neuron-specific protein kinase C substrate (post synaptic protein) whose main function is to bind CaM (calmodulin) in the absence of Ca2+. Neurogranin modulates the homeostasis of Ca2+ in neurons and its role has been implicated in learning and memory.
- Ligand/Receptor
- Calmodulin-binding.
- Cell Type
- Neurons
- Biology Area
- Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Antigen References
-
1. Zhong L, et al. 2015. J. Neurosci. 35:7503.
2. Huang K, et al. 2004. J. Neurosci. 24:10660.
3. Dominguez-Gonzalez I, et al. 2007. Biochem. J. 404:31.
4. Prichard L, et al. 1999. J. Biol. Chem. 274:7689. - Gene ID
- 4900 View all products for this Gene ID
- UniProt
- View information about Neurogranin on UniProt.org
Related FAQs
Other Formats
View All Neurogranin (NRGN) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Neurogranin (NRGN) | NG7 | IHC-P,WB,IP,ELISA Capture |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-Neurogranin (NRGN)
IHC staining of Clone NG7 on formalin fixed paraffin embedde... 
Western blot analysis of Clone NG7. Lane 1: Molecular weight...
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